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The potential of Bacillus sp. and Fusarium sp. as biosorbents for the removal of copper and iron from contaminated samples was evaluated under varying conditions, including pH, metal concentration, and temperature, to optimize the biosorption process. Both microbial strains exhibited high biosorption efficiencies, with removal percentages varying based on exposure duration. The study's findings highlight the effectiveness of Bacillus sp. and Fusarium sp. in significantly reducing copper and iron concentrations in both wastewater and soil, offering a sustainable and eco-friendly approach to heavy metal remediation. The use of these microbial biosorbents presents a promising alternative for mitigating environmental pollution caused by industrial waste, contributing to improved soil and water quality while reducing reliance on conventional chemical treatments.