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The study of the effects of environmental pollutants on human health has received widespread attention, including whether traditional pollutants such as particulate matters and heavy metals, or new pollutants such as nanoparticles and microplastics can cause serious impact to the environment and human health. Research on the potential toxic effects and mechanisms of environmental pollutants can provide a scientific basis for related prevention work. The purpose of this Special Issue is to publish research on the potential toxicity and mechanisms of environmental pollutants affecting humans or the environment. We encourage the use of novel methods such as multi-omics techniques, or research on new pollutants such as nanomaterials. Research using biomarkers to conduct biological monitoring to assess the impact of environmental or occupational pollutants on human exposure or health is also included, as well as reviews summarizing relevant recent advances.