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Malaria, dengue and lymphatic filariasis are diseases transmitted by Anopheles, Aedes and Culex mosquitoes, respectively. These mosquitoes have evolved and adapted to environmental conditions and human lifestyles, providing them with a variety of breeding sites. This study aimed to determine the influence of the physicochemical characteristics of breeding sites on the distribution of Anopheles, Aedes and Culex mosquitoes in Benin.This study highlights the importance of the physicochemical properties of breeding sites in the distribution of primary vector mosquito species. The prevalence of domestic containers and tires as breeding sites underscores the necessity of targeted interventions in these habitats, both indoors and outdoors of human dwellings.