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This book is a part of PhD Thesis work entitled "Lactose malabsorption in irritable bowel syndrome in the tropics: evaluation of diagnostic protocol, study of LPH & SERT genes potentially influencing development of symptoms following lactose ingestion". We have evaluated the different techniques of diagnosis of lactose intolerance like Lactose Tolerance Test, Lactose Hydrogen Breath Test, Lactase genotyping for hypolactsia. We have also studied the role serotonin transporter (SERT) gene in symptom development following different doses of lactose. This book will be good collection of lactose intolerance diagnosis and its treatment. Also, we have evaluated the role of lactose intolerance in patients with Irritable Bowel Syndrome. All the clinician treating lactose intolerance and Irritable Bowel Syndrome are invited to read this book.