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In recent years, peptides generated from various marine organisms have attracted wide attention due to their diverse chemical structures and bioactivities, including antioxidant, immune-modulating, angiotensin-I-converting enzyme (ACE)-inhibitory, hypotensive, hypolipidemic, antiviral, cytotoxic, neurotoxic, anticoagulant, and antifreeze activities. Moreover, bioactive peptides can serve as additional functional components used in health foods, nutraceuticals, bio-preservatives, cosmetics, and pharmaceuticals. The Special Issue "Marine Bioactive Peptides-Structure, Function, and Application 2.0" aimed to collect papers on up-to-date information regarding the preparation, structural identification, activity, and functional evaluation, as well as the application of marine bioactive peptides.