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HLA-E(Class 1) is a MHC gene which has been isolated in 2020, in our laboratory. We show now its biosynthesis « de novo » in a PUC-GW-KAN plasmid. Such experiment was performed with the Ophiocomina nigra IGKappa gene one year ago.We have isolated recently MHC genes in Echinodermata ( Ref 1) in 3 classes: the Ophuirids, the Crinoïds, the Asterids. At that time we decided to synthetise one of these genes: the well-known HLA-E one in a PUC-GW-KAN plasmid (Yan Li gift).