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A series of novel 2-(substituted benzylidene)-7-(4-bromophenyl)-5-(furan-2-yl)-2H-thiazolo[3,2-a]pyrimidin-3(7H)-ones were synthesized by using established methods and were characterized by IR, 1H NMR and Mass spectroscopy in order to confirm their formation. These compounds were synthesized to evaluate their antitubercular, antibacterial and antifungal potency. Docking studies revealed the importance of functional groups and its different biological action. They were found to be potent against Mycobacterium tuberculosis H37Ra strain, also potent against Gram +ve, Gram -ve and fungal strains