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The genus Bordetella contains several pathogenic
species generally associated with upper respiratory
tract infections in warm-blooded animals. Bordetella
pertussis is the etiologic agent of whooping cough.
Whooping cough is presently one of the ten most
common causes of death from infectious diseases and
reported by the World Health Organisation (WHO) to
cause 50 million cases and 350000 deaths worldwide
per year.
The term proteome, in analogy to the term genome, was
coined to describe the complete set of proteins that
an organism has produced under a defined set of
conditions. Proteomics has been used to identify
novel bacterial vaccine candidates against several
human pathogens. Much of information about
immunogenic component can be derived from proteomics
coupled to Western blotting, namely immunoproteomics.
In the present study, we report first
immunoproteomics analysis to identify candidate
antigens of B. pertussis for vaccine development.
Different sera from mice, which were immunized or
challenged with B. pertussis, were analyzed for
reactivity by Western blot against whole cell
extracts of B. pertussis Tohama and Saadet strains
separated by 2-DE.
species generally associated with upper respiratory
tract infections in warm-blooded animals. Bordetella
pertussis is the etiologic agent of whooping cough.
Whooping cough is presently one of the ten most
common causes of death from infectious diseases and
reported by the World Health Organisation (WHO) to
cause 50 million cases and 350000 deaths worldwide
per year.
The term proteome, in analogy to the term genome, was
coined to describe the complete set of proteins that
an organism has produced under a defined set of
conditions. Proteomics has been used to identify
novel bacterial vaccine candidates against several
human pathogens. Much of information about
immunogenic component can be derived from proteomics
coupled to Western blotting, namely immunoproteomics.
In the present study, we report first
immunoproteomics analysis to identify candidate
antigens of B. pertussis for vaccine development.
Different sera from mice, which were immunized or
challenged with B. pertussis, were analyzed for
reactivity by Western blot against whole cell
extracts of B. pertussis Tohama and Saadet strains
separated by 2-DE.