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Many synthesis pathways were designed to study the activity of Fructose-6-phosphate and Glycerol dehydrogenase. In this work the synthesis of glycerine through these enzymes were studied.Fructose-6-phosphate aldolase cleavages fructose-6-phophate to glyceraldehyde-3-phosphate and dihydroxyacetone. To produce glycerin from dihydroxyacetone, glycerol dehydrogenase was used.In this work, a mutant of Fructose-6-phosphate aldolase is used, called Fructose-6-phosphate aldolase A129S, which it efficiency is higher.