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Increase in population leads toward industrialization to meet demands of the country. Pharmaceutical industry being major sector plays an important role in the economic growth of Pakistan but on other hand, it is contributing in environmental pollution. Different physico-chemical parameters like pH, BOD, COD, TDS, TSS and turbidity were analyzed in waste water samples of industry. Trace hazardous pollutants were also identified in effluent by using High Pressure Liquid Chromatography (HPLC) and Thin Layer Chromatography (TLC).