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The aim of the study was to evaluate antifungal activity of Padina tetrastromatica from sandspit, Karachi coast belong to phylum phaeophyta. Four concentrations were made along with crude extract of alga in different solvents to test for their actions against the fungi species which are pathogenic to cause various diseases. A control with other match able concentrations was also tested in experiments. The results of the experiments were excellent on the ability of antifungal activity. The most stricking results were shown by Chloroform extract against Trichoderma sp. which were 40.3±0.38 mm by crude, 32.1±0.55 mm by 1/1 concentration. The least active extract was n-Hexane and methanol against Aspergillus niger, Botrytis sp. and Rhizopus stolonifer. It was also noted that chloroform extract was most effective against all types of species with excellent result. Aqueous extract showed zero activity against all type of fungi expect Penicillium notatum which showed least activity. N-Hexane extract of algae was considerably a strong antifungal agent against Penicillium notatum in its all concentrations. However, n-Hexane extract with 1/100 (g/ml) showed nothing against Aspergillus niger.