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Leaf-cutting ants of the genera Atta and Acromyrmex are considered important pests in the Neotropics. Currently, the main control method employed is the use of chemical insecticides. However, there is growing concern about the negative effects of frequent insecticide applications on non-target organisms, human health and the environment. Entomopathogenic fungi can naturally infect host insects via direct penetration of the cuticle and have been studied worldwide as promising biological control agents.