Tim Wehr (ed.)

Capillary Electrophoresis of Proteins

Herausgeber: Wehr, Tim; Zhu, Mingde; Rodriguez-Diaz, Roberto

• Gebundenes Buch

Produktbeschreibung

"Provides practical information on the application of capillary electrophoresis (CE) to protein analysis, with an emphasis on developing and optimizing CE techniques in the laboratory. Includes separation methods bases on mass, charge, isoelectric point, molecular sieving, and affinity interactions."

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Produktdetails

• Verlag: CRC Press
• Seitenzahl: 302
• Erscheinungstermin: 20. Oktober 1998
• Englisch
• Abmessung: 235mm x 157mm x 21mm
• Gewicht: 591g
• ISBN-13: 9780824702052
• ISBN-10: 0824702050
• Artikelnr.: 22298210

Autorenporträt

Tim Wehr, Roberto Rodriguez-Diaz, Mingde Zhu

Inhaltsangabe

Principles and practice of capillary electrophoresis: basic concepts
brand broadening in capillary electrophoresis
capillary electrophoresis intrumentation
preparative capillary electrophoresis
capillary electrophoresis separation modes. Detection of proteins: absorbance detection
fluorescence detection
mass spectrometry. Strategies for reducing protein-wall interactions: operations at pH extremes
use of buffer additives
capillary coatings and other surface modifications. Capillary zone electrophoresis: developing a CZE method
sample preconcentration techniques
applications. Capillary isoelectric focusing: sample preparation and injection
focusing
two-step CIEF
single-step CIEF
capillary selection
detection
optimizing CIEF analysis
capillary IEF as a micro-preparative technique
applications
future developments. Sieving separation: analysis of native proteins
analysis of SDS-protein complexes. Concluding remarks.