Chemokines, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in chemokines, and includes sections on such topics as chemokine detection using receptors, tracking cellular responses to chemokines, recognition of GAG-bound chemokines, and the production of chemokine receptor complexes for structural and biophysical studies.
Chemokines, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in chemokines, and includes sections on such topics as chemokine detection using receptors, tracking cellular responses to chemokines, recognition of GAG-bound chemokines, and the production of chemokine receptor complexes for structural and biophysical studies.
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Inhaltsangabe
1. Chemokine Detection Using Receptors Immobilized on an Spr Sensor Surface José Miguel Rodríguez-Frade, Laura Martínez-Muñoz, Ricardo Villares, Graciela Cascio, Pilar Lucas, Rosa P. Gomariz and Mario Mellado 2. Study of Chemotaxis and Cell-Cell Interactions in Cancer with Microfluidic Devices Jiqing Sai, Matthew Rogers, Kathryn Hockemeyer, John P. Wikswo and Ann Richmond 3. Generating Chemokine Analogues with Enhanced Pharmacological Properties using Phage Display Karim Dorgham, Fabrice Cerini, Hubert Gaertner, Astrid Melotti, Irène Rossitto- Borlat, Guy Gorochov and Oliver Hartley 4. Methods for the Recognition of GAG-Bound Chemokines Pauline Bonvin, Nicolas Fischer and Amanda Proudfoot 5. Monitoring Scavenging Activity of Chemokine Receptors Barbara Moepps and Marcus Thelen 6. Dual Color Luciferase Complementation for Chemokine Receptor Signaling Kathryn E. Luker and Gary D. Luker 7. Analysis of Arrestin Recruitment to Chemokine Receptors by Bioluminescence Resonance Energy Transfer J. Bonneterre, N. Montpas, C. Boularan, C. Galés and N. Heveker 8. Probing Biased Signaling in Chemokine Receptors Roxana-Maria Amarandi, Gertrud Malene Hjortø, Mette Marie Rosenkilde and Stefanie Karlshøj 9. Mutagenesis by Phage Display Pauline Bonvin, Christine Power, Amanda Proudfoot and Steven Dunn 10. Studying Chemokine Control of Neutrophil Migration in Vivo in a Murine Model of Inflammatory Arthritis Yoshishige Miyabe, Nancy D. Kim, Chie Miyabe, and Andrew D. Luster 11. Production of Chemokine/Chemokine Receptor Complexes for Structural and Biophysical Studies Martin Gustavsson, Yi Zheng and Tracy M. Handel 12. In Vivo Models to Study Chemokine Biology F.A. Amaral, D. Boff and M.M. Teixeira 13. Monitoring Chemokine Receptor Trafficking by Confocal Immunofluorescence Microscopy Adriano Marchese 14. Active Shaping of Chemokine Gradients by Atypical Chemokine Receptors: a 4D live Cell Imaging Migration Assay Kathrin Werth and Reinhold Förster 15. The Role of Chemokine and Glycosaminoglycan Interaction in Chemokine- Mediated Migration in Vitro and in Vivo Irene del Molino del Barrio, John Kirby and Simi Ali 16. Examining Roles of Glycans in Chemokine-Mediated Dendritic-Endothelial Cell Interactions Xin Yin, Scott C. Johns, Roland El Ghazal, Catherina L. Salanga, Tracy M. Handel and Mark M. Fuster 17. Preparation and Analysis of N-terminal Chemokine Receptor Sulfopeptides Using Tyrosylprotein Sulfotransferase Enzymes Christoph Seibert, Anthony Sanfiz, Thomas P. Sakmar, and Christopher T. Veldkamp 18. Disulfide Trapping for Modeling and Structure Determination of Receptor:Chemokine Complexes Irina Kufareva, Martin Gustavsson, Lauren G. Holden, Ling Qin, Yi Zheng and Tracy M. Handel 19. Analysis of G Protein and ?-Arrestin Activation in Chemokine Receptors Signaling Alessandro Vacchini, Marta Busnelli, Bice Chini, Massimo Locati and Elena Monica Borroni 20. Flow Cytometry Detection of Chemokine Receptors for the Identification of Murine Monocyte and Neutrophil Subsets Ornella Bonavita, Matteo Massara, Achille Anselmo, Paolo Somma, Hilke Brühl, Matthias Mack, Massimo Locati and Raffaella Bonecchi 21. Molecular Pharmacology of Chemokine Receptors Raymond H. de Wit, Sabrina M. de Munnik, Rob Leurs, Henry F. Vischer and Martine J. Smit 22. Preparation and Characterization of Glycosaminoglycan Chemokine Co-Receptors Nikola Kitic, Martha Gschwandtner, Rupert Derler, Tanja Gerlza and Andreas J. Kungl 23. Production of Recombinant Chemokines and Validation of Refolding Christopher T. Veldkamp, Chad A. Koplinski, Davin R. Jensen, Francis C. Peterson, Kaitlin M. Smits, Brittney L. Smith, Scott K. Johnson, Christina Lettieri, Wallace G. Buchholz,Joyce C. Solheim and Brian F. Volkman 24. Quantitative Analysis of Dendritic Cell Haptotaxis Jan Schwarz, Michael Sixt
1. Chemokine Detection Using Receptors Immobilized on an Spr Sensor Surface José Miguel Rodríguez-Frade, Laura Martínez-Muñoz, Ricardo Villares, Graciela Cascio, Pilar Lucas, Rosa P. Gomariz and Mario Mellado 2. Study of Chemotaxis and Cell-Cell Interactions in Cancer with Microfluidic Devices Jiqing Sai, Matthew Rogers, Kathryn Hockemeyer, John P. Wikswo and Ann Richmond 3. Generating Chemokine Analogues with Enhanced Pharmacological Properties using Phage Display Karim Dorgham, Fabrice Cerini, Hubert Gaertner, Astrid Melotti, Irène Rossitto- Borlat, Guy Gorochov and Oliver Hartley 4. Methods for the Recognition of GAG-Bound Chemokines Pauline Bonvin, Nicolas Fischer and Amanda Proudfoot 5. Monitoring Scavenging Activity of Chemokine Receptors Barbara Moepps and Marcus Thelen 6. Dual Color Luciferase Complementation for Chemokine Receptor Signaling Kathryn E. Luker and Gary D. Luker 7. Analysis of Arrestin Recruitment to Chemokine Receptors by Bioluminescence Resonance Energy Transfer J. Bonneterre, N. Montpas, C. Boularan, C. Galés and N. Heveker 8. Probing Biased Signaling in Chemokine Receptors Roxana-Maria Amarandi, Gertrud Malene Hjortø, Mette Marie Rosenkilde and Stefanie Karlshøj 9. Mutagenesis by Phage Display Pauline Bonvin, Christine Power, Amanda Proudfoot and Steven Dunn 10. Studying Chemokine Control of Neutrophil Migration in Vivo in a Murine Model of Inflammatory Arthritis Yoshishige Miyabe, Nancy D. Kim, Chie Miyabe, and Andrew D. Luster 11. Production of Chemokine/Chemokine Receptor Complexes for Structural and Biophysical Studies Martin Gustavsson, Yi Zheng and Tracy M. Handel 12. In Vivo Models to Study Chemokine Biology F.A. Amaral, D. Boff and M.M. Teixeira 13. Monitoring Chemokine Receptor Trafficking by Confocal Immunofluorescence Microscopy Adriano Marchese 14. Active Shaping of Chemokine Gradients by Atypical Chemokine Receptors: a 4D live Cell Imaging Migration Assay Kathrin Werth and Reinhold Förster 15. The Role of Chemokine and Glycosaminoglycan Interaction in Chemokine- Mediated Migration in Vitro and in Vivo Irene del Molino del Barrio, John Kirby and Simi Ali 16. Examining Roles of Glycans in Chemokine-Mediated Dendritic-Endothelial Cell Interactions Xin Yin, Scott C. Johns, Roland El Ghazal, Catherina L. Salanga, Tracy M. Handel and Mark M. Fuster 17. Preparation and Analysis of N-terminal Chemokine Receptor Sulfopeptides Using Tyrosylprotein Sulfotransferase Enzymes Christoph Seibert, Anthony Sanfiz, Thomas P. Sakmar, and Christopher T. Veldkamp 18. Disulfide Trapping for Modeling and Structure Determination of Receptor:Chemokine Complexes Irina Kufareva, Martin Gustavsson, Lauren G. Holden, Ling Qin, Yi Zheng and Tracy M. Handel 19. Analysis of G Protein and ?-Arrestin Activation in Chemokine Receptors Signaling Alessandro Vacchini, Marta Busnelli, Bice Chini, Massimo Locati and Elena Monica Borroni 20. Flow Cytometry Detection of Chemokine Receptors for the Identification of Murine Monocyte and Neutrophil Subsets Ornella Bonavita, Matteo Massara, Achille Anselmo, Paolo Somma, Hilke Brühl, Matthias Mack, Massimo Locati and Raffaella Bonecchi 21. Molecular Pharmacology of Chemokine Receptors Raymond H. de Wit, Sabrina M. de Munnik, Rob Leurs, Henry F. Vischer and Martine J. Smit 22. Preparation and Characterization of Glycosaminoglycan Chemokine Co-Receptors Nikola Kitic, Martha Gschwandtner, Rupert Derler, Tanja Gerlza and Andreas J. Kungl 23. Production of Recombinant Chemokines and Validation of Refolding Christopher T. Veldkamp, Chad A. Koplinski, Davin R. Jensen, Francis C. Peterson, Kaitlin M. Smits, Brittney L. Smith, Scott K. Johnson, Christina Lettieri, Wallace G. Buchholz,Joyce C. Solheim and Brian F. Volkman 24. Quantitative Analysis of Dendritic Cell Haptotaxis Jan Schwarz, Michael Sixt
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Praise for the Series: "Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry "The work most often consulted in the lab." --Enzymologia "The Methods in Enzymology series represents the gold-standard." --Neuroscience
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