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Butea monosperma (Lamk.) Taub. is one such medicinal plant commonly known as Palas (Flame of the forest) belonging to the family Fabaceae. It is distributed throughout India, except arid parts (Sharma et al., 2000). Butea monosperma (Lamk.) Taub. has various healing effects which are seen in treatment of many diseases. The information of Butea monosperma (Lamk.) Taub. is very meagre and hence it was thought of interest to undertake the present work to study antidiabetic, antimicrobial and antioxidant property of different plant parts of Butea monosperma (Lamk.) Taub.Anatomical features were studied by taking hand sections. Preliminary phytochemical analysis was carried for leaf, bark and flowers of Butea monosperma (Lamk.) Taub. TLC and HPTLC analysis was also performed. Colour dye from flowers was extracted using different polar and nonpolar solvents. Acute toxicity and Efficacy study of Butea monosperma (Lamk.) Taub. was carried out on Animal model as per OECD guidelines.Antimicrobial activity of leaf, bark and flower of Butea monosperma (Lamk.) Taub. was evaluated by using agar well diffusion method using Mueller Hinton agar against some gram positive and gram negative bacteria.