Part I. Background Information: 1. Culture of plant cells, tissues, and organs 2. Laboratory facilities 3. Aseptic techniques 4. Media composition and preparation Part II. Experimental: Callus and Callus-Derived Systems: 5. Initiation and maintenance of callus 6. Organogenesis 7. Cell suspensions 8. Somatic embryogenesis Part III. Experimental: Culture of Organs and Organized Systems: 9. Isolated roots 10. Micropropagation by bud proliferation 11. Anther and pollen cultures Part IV. Experimental: Isolated Cells: 12. Transdifferentiation of parenchyma cells to tracheary elements 13. Isolation and culture of protoplasts 14. Protoplast fusion and somatic hybridization Part V. Supplementary Topics: 15. Cryopreservation of germplasm 16. Production of secondary metabolites 17. Quantitation of procedures 18. Formulations of tissue culture media 19. Commercial sources of supplies Author index Subject index.
Part I. Background Information: 1. Culture of plant cells, tissues, and organs 2. Laboratory facilities 3. Aseptic techniques 4. Media composition and preparation Part II. Experimental: Callus and Callus-Derived Systems: 5. Initiation and maintenance of callus 6. Organogenesis 7. Cell suspensions 8. Somatic embryogenesis Part III. Experimental: Culture of Organs and Organized Systems: 9. Isolated roots 10. Micropropagation by bud proliferation 11. Anther and pollen cultures Part IV. Experimental: Isolated Cells: 12. Transdifferentiation of parenchyma cells to tracheary elements 13. Isolation and culture of protoplasts 14. Protoplast fusion and somatic hybridization Part V. Supplementary Topics: 15. Cryopreservation of germplasm 16. Production of secondary metabolites 17. Quantitation of procedures 18. Formulations of tissue culture media 19. Commercial sources of supplies Author index Subject index.
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