Comprehensive and up-to-date, here experienced and outstanding international experts offer a one-stop reference on the principles and applications of this highly versatile separation technology. Following introductory chapters on the theory, basic principles and instrumentation, a tutorial-style user guide addresses typical users' questions and problems. In the remaining part, the separation of all relevant particle classes is discussed, covering the latest developments in instrumentation as well as applications. As a result, both beginners and experienced researchers can confidently rely on…mehr
Comprehensive and up-to-date, here experienced and outstanding international experts offer a one-stop reference on the principles and applications of this highly versatile separation technology. Following introductory chapters on the theory, basic principles and instrumentation, a tutorial-style user guide addresses typical users' questions and problems. In the remaining part, the separation of all relevant particle classes is discussed, covering the latest developments in instrumentation as well as applications. As a result, both beginners and experienced researchers can confidently rely on this book to confirm their own understanding or to improve their field-flow fractionation methods and the interpretation of the results.
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Autorenporträt
Céline Guéguen, PhD, is Full Professor of Chemistry at the Université de Sherbrooke, Québec, Canada.
Mohammed Baalousha, PhD, is Professor of Environmental Nanoscience and director of the Environmental Nanoscience & Analytics Laboratory at the University of South Carolina, Columbia, South Carolina, United States.
S. Kim R. Williams is a Professor of Chemistry at the Colorado School of Mines, Golden, Colorado, United States .
Inhaltsangabe
PART I: THEORY Chapter 1. The Basics FFF Overview Historical perspectives Normal vs steric vs hyperlayer modes Retention Resolution, fractionation power Chapter 2. FFF techniques AF4 - Flow FFF (asymmetric, frit inlet and outlet) HF5- Hollow-fiber flow FFF Sedimentation FFF Thermal FFF Electrical FFF Novel techniques (including 2D-FFF) Chapter 3. User guide Which FFF is right for you? How to use FF correctly? General optimization strategies: carrier fluid, calibration, reproducibility Sample overloading Sample recovery Which detector to choose? How to troubleshoot FFF? (in general terms) Chapter 4. Other physical fractionation techniques: focus on performance as compared to the FFF Filtration/Ultrafiltration Centrifugation/ultracentrifugation Size exclusion chromatography Liquid chromatography Microfluidics Precipitation
PART II: APPLICATIONS Chapter 5. Polymer and latex Chapter 6. Biological particles: Virus, DNA/RNA, drug delivery Chapter 7. Nanoparticles in water and soil Chapter 8. Manufactured nanoparticles Chapter 9. Protein, enzymes Chapter 10. Cell separations - blood and algal cells Chapter 11. Food industry - Starch Chapter 12. Pharmaceutical industry - drug delivery Chapter 13. Disease diagnosis
PART I: THEORY Chapter 1. The Basics FFF Overview Historical perspectives Normal vs steric vs hyperlayer modes Retention Resolution, fractionation power Chapter 2. FFF techniques AF4 - Flow FFF (asymmetric, frit inlet and outlet) HF5- Hollow-fiber flow FFF Sedimentation FFF Thermal FFF Electrical FFF Novel techniques (including 2D-FFF) Chapter 3. User guide Which FFF is right for you? How to use FF correctly? General optimization strategies: carrier fluid, calibration, reproducibility Sample overloading Sample recovery Which detector to choose? How to troubleshoot FFF? (in general terms) Chapter 4. Other physical fractionation techniques: focus on performance as compared to the FFF Filtration/Ultrafiltration Centrifugation/ultracentrifugation Size exclusion chromatography Liquid chromatography Microfluidics Precipitation
PART II: APPLICATIONS Chapter 5. Polymer and latex Chapter 6. Biological particles: Virus, DNA/RNA, drug delivery Chapter 7. Nanoparticles in water and soil Chapter 8. Manufactured nanoparticles Chapter 9. Protein, enzymes Chapter 10. Cell separations - blood and algal cells Chapter 11. Food industry - Starch Chapter 12. Pharmaceutical industry - drug delivery Chapter 13. Disease diagnosis
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