Flow cytometry plays a critical role in the diagnosis of hematopoietic neoplasms. Understanding the key immunophenotypic features of each entity is essential for accurate diagnosis. Filling a crucial gap in current literature, this is a comprehensive reference text that systematically details these immunophenotypic profiles. Grounded in real-world experience, this practice-driven resource offers expert guidance on the use of flow cytometry in the diagnosis and classification of hematolymphoid neoplasms. Covering major disease categories, technical insights, and emerging therapies like CAR…mehr
Flow cytometry plays a critical role in the diagnosis of hematopoietic neoplasms. Understanding the key immunophenotypic features of each entity is essential for accurate diagnosis. Filling a crucial gap in current literature, this is a comprehensive reference text that systematically details these immunophenotypic profiles. Grounded in real-world experience, this practice-driven resource offers expert guidance on the use of flow cytometry in the diagnosis and classification of hematolymphoid neoplasms. Covering major disease categories, technical insights, and emerging therapies like CAR T-cells, readers will gain a solid foundation for identifying deviations seen in hematologic malignancies, thereby enhancing diagnostic accuracy. Each chapter concludes with key points and diagnostic pitfalls for efficient comprehension and retention. This is an essential tool for pathologists, hematologists, trainees, and laboratory professionals seeking clarity and confidence in diagnosis.
Section I. Mature B-Cell and Plasma Cell Neoplasms; 1. B-Cells: normal maturation and strategies in identification of abnormal cells Wei Wang; 2. Chronic lymphocytic leukemia/small lymphocytic lymphoma and mantle cell lymphoma Wei Wang; 3. Follicular lymphoma Wei Wang; 4. Low-grade b-cell lymphomas with plasmacytic differentiation Andres E Quesada and Pei Lin; 5. Splenic b-cell lymphoma/leukemia (other than splenic marginal zone lymphoma) and b-cell prolymphocytic leukemia Beenu Thakral and Sa A Wang; 6. Aggressive b-cell lymphomas Shaoying Li, Jie Xu and Lianqun Qiu; 7. Large b-cell lymphoma with plasmablastic differentiation Lianqun Qiu, Shaoying Lin, Pei Lin, Jiehao Zhou, Yi Zhou and Jie Xu; 8. Plasma cell neoplasms Andres E Quesada and Pei Lin; Section II. Mature T-Cell and Nk-Cell Neoplasms; 9. T and NK cells: normal maturation and strategies in identification of abnormal cells Wei Wang; 10. T-cell large granular lympocytic leukemia Min Shi, Michael M. Timm and Dragan Jevremovic; 11. Hepatosplenic T-cell lymphoma Min Shi, Michael M. Timm and Dragan Jevremovic; 12. Adult T-cell leukemia/lymphoma Hong Fang and Wei Wang; 13. T-prolymphocytic leukemia Hong Fang and Wei Wang; 14. Mycosis fungoides/ Sezary syndrome Hong Fang and Wei Wang; 15. NK-Large granular lympocytic leukemia Min Shi, Michael M. Timm and Dragan Jevremovic; 16. Aggressive NK-cell leukemia Min Shi, Michael M. Timm and Dragan Jevremovic; 17. Nodal T follicular helper cell lymphoma Chi Young Ok, Francisco Vega and Sa A. Wang; 18. Anaplastic large cell lymphoma, ALK-positive Jie Xu, Shaoying Li and L. Jeffrey Medeiros; 19. Anaplastic large cell lymphoma, ALK-negative Jie Xu, Jeffrey Medeiros and Shaoying Li; 20. Other nodal and extranodal mature T/NK-cell lymphomas Chi Young Ok, Francisco Vega and Sa A. Wang; Section III. Acute Lymphoblastic Leukemia/Lymphoma; 21. B-lymphopblastic leukemia/lymphoma Sa A. Wang; 22. T-lymphopblastic leukemia/lymphoma Sa A. Wang; Section IV. Acute Myeloid Leukemia; 23. Acute myeloid leukemia: introduction Wei Wang; 24. Acute myeloid leukemia with RUNX1: RUNX1T1 fusion Wei Wang; 25. Acute myeloid leukemia with CBFB rearrangement Wei Wang; 26. Acute promyelocytic leukemia Hong Fang and Wei Wang; 27. Acute myeloid leukemia with NPM1 mutation Wei Wang; 28. Acute myeloid leukemia with KMT2A rearrangement Wei Wang; 29. Acute myeloid leukemia with MECOM rearrangement Wei Wang; 30. Acute myeloid leukemia with DEK: NUP214 fusion Wei Wang; 31. Acute myeloid leukemia with NUP98 rearrangement Mahsa Khanlari and Wei Wang; 32. Acute myeloid leukemia with mutated TP53 Fnu Sameeta and Wei Wang; 33. Acute erythroid leukemia Wei Wang; 34. Acute megakaryoblastic leukemia Mahsa Khanlari and Wei Wang; 35. Normal myelomonocytic maturation and evaluation of acute myeloid leukemia minimal/measurable residual disease Wei Wang and Sa A. Wang; Section V. Acute Leukemias of Mixed or Ambiguous Lineage; 36. Acute undifferentiated leukemia Vasiliki Leventaki and Sa A. Wang; 37. Mixed-phenotype acute leukemia Vasiliki Leventaki and Sa A Wang; Section VI. Neoplastic Plasmacytoid Dendritic Cell Proliferation; 38. Plasmacytoid dendritic cells: from normal maturation to neoplastic proliferations Wei Wang; Section VII. Other Myeloid Neoplasms and Bone Marrow Failure Diseases; 39. Chronic myeloid neoplasms and bone marrow failure disorders Sanam Loghavi, Sa A. Wang and Wei Wang; 40. Systemic mastocytosis Wei Wang, Jie Xu, Sa A. Wang, Lianqun Qiu and Shaoying Li; Section VIII. Flow Cytometry Data Handling; 41. Flow cytometry data processing, analysis, artifacts and pitfalls Xuehai Wang; Section IX. Chimeric Antigen Receptor (Car) T-Cell; 42. Chimeric antigen receptor (CAR) T-Cell immunotherapy and flow cytometric monitoring of CAR T-cells Jianhua Ling and Sa A. Wang; Appendix: common flow cytometry markers and their utility.
Section I. Mature B-Cell and Plasma Cell Neoplasms; 1. B-Cells: normal maturation and strategies in identification of abnormal cells Wei Wang; 2. Chronic lymphocytic leukemia/small lymphocytic lymphoma and mantle cell lymphoma Wei Wang; 3. Follicular lymphoma Wei Wang; 4. Low-grade b-cell lymphomas with plasmacytic differentiation Andres E Quesada and Pei Lin; 5. Splenic b-cell lymphoma/leukemia (other than splenic marginal zone lymphoma) and b-cell prolymphocytic leukemia Beenu Thakral and Sa A Wang; 6. Aggressive b-cell lymphomas Shaoying Li, Jie Xu and Lianqun Qiu; 7. Large b-cell lymphoma with plasmablastic differentiation Lianqun Qiu, Shaoying Lin, Pei Lin, Jiehao Zhou, Yi Zhou and Jie Xu; 8. Plasma cell neoplasms Andres E Quesada and Pei Lin; Section II. Mature T-Cell and Nk-Cell Neoplasms; 9. T and NK cells: normal maturation and strategies in identification of abnormal cells Wei Wang; 10. T-cell large granular lympocytic leukemia Min Shi, Michael M. Timm and Dragan Jevremovic; 11. Hepatosplenic T-cell lymphoma Min Shi, Michael M. Timm and Dragan Jevremovic; 12. Adult T-cell leukemia/lymphoma Hong Fang and Wei Wang; 13. T-prolymphocytic leukemia Hong Fang and Wei Wang; 14. Mycosis fungoides/ Sezary syndrome Hong Fang and Wei Wang; 15. NK-Large granular lympocytic leukemia Min Shi, Michael M. Timm and Dragan Jevremovic; 16. Aggressive NK-cell leukemia Min Shi, Michael M. Timm and Dragan Jevremovic; 17. Nodal T follicular helper cell lymphoma Chi Young Ok, Francisco Vega and Sa A. Wang; 18. Anaplastic large cell lymphoma, ALK-positive Jie Xu, Shaoying Li and L. Jeffrey Medeiros; 19. Anaplastic large cell lymphoma, ALK-negative Jie Xu, Jeffrey Medeiros and Shaoying Li; 20. Other nodal and extranodal mature T/NK-cell lymphomas Chi Young Ok, Francisco Vega and Sa A. Wang; Section III. Acute Lymphoblastic Leukemia/Lymphoma; 21. B-lymphopblastic leukemia/lymphoma Sa A. Wang; 22. T-lymphopblastic leukemia/lymphoma Sa A. Wang; Section IV. Acute Myeloid Leukemia; 23. Acute myeloid leukemia: introduction Wei Wang; 24. Acute myeloid leukemia with RUNX1: RUNX1T1 fusion Wei Wang; 25. Acute myeloid leukemia with CBFB rearrangement Wei Wang; 26. Acute promyelocytic leukemia Hong Fang and Wei Wang; 27. Acute myeloid leukemia with NPM1 mutation Wei Wang; 28. Acute myeloid leukemia with KMT2A rearrangement Wei Wang; 29. Acute myeloid leukemia with MECOM rearrangement Wei Wang; 30. Acute myeloid leukemia with DEK: NUP214 fusion Wei Wang; 31. Acute myeloid leukemia with NUP98 rearrangement Mahsa Khanlari and Wei Wang; 32. Acute myeloid leukemia with mutated TP53 Fnu Sameeta and Wei Wang; 33. Acute erythroid leukemia Wei Wang; 34. Acute megakaryoblastic leukemia Mahsa Khanlari and Wei Wang; 35. Normal myelomonocytic maturation and evaluation of acute myeloid leukemia minimal/measurable residual disease Wei Wang and Sa A. Wang; Section V. Acute Leukemias of Mixed or Ambiguous Lineage; 36. Acute undifferentiated leukemia Vasiliki Leventaki and Sa A. Wang; 37. Mixed-phenotype acute leukemia Vasiliki Leventaki and Sa A Wang; Section VI. Neoplastic Plasmacytoid Dendritic Cell Proliferation; 38. Plasmacytoid dendritic cells: from normal maturation to neoplastic proliferations Wei Wang; Section VII. Other Myeloid Neoplasms and Bone Marrow Failure Diseases; 39. Chronic myeloid neoplasms and bone marrow failure disorders Sanam Loghavi, Sa A. Wang and Wei Wang; 40. Systemic mastocytosis Wei Wang, Jie Xu, Sa A. Wang, Lianqun Qiu and Shaoying Li; Section VIII. Flow Cytometry Data Handling; 41. Flow cytometry data processing, analysis, artifacts and pitfalls Xuehai Wang; Section IX. Chimeric Antigen Receptor (Car) T-Cell; 42. Chimeric antigen receptor (CAR) T-Cell immunotherapy and flow cytometric monitoring of CAR T-cells Jianhua Ling and Sa A. Wang; Appendix: common flow cytometry markers and their utility.
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