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Nowadays, Herbal ingredients have become globally significant in hair care products like hair serums, hair shampoo etc. for both of their medicinal and economical value. This study presents the formulation and evaluation of Poly Herbal hair growth serum composed of natural ingredients including Hibiscus rosa-sinesis, Aloe Vera, Flaxseeds, almond extract and Japanese Cherry Blossom. It is basically hair care product in liquid form; its consistency is thicker than water. This serum is designed to promote the healthy growth of hair, reduce the hair breakage, hair fall, dandruff & enhance the overall scalp health. This formulation emphasizes the natural, untoxic, chemical free approaches to the hair care. All the parameters were found to be good and within the standards. The main objective of the present work is to develop a Poly herbal hair serum for general purpose using various herbs with this evaluate the characteristics of cosmetic serum and to identify the best formulation of cosmetic serum.