The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences. Methods in Enzymology is now available online at ScienceDirect - full-text online of volumes 1 onwards. For more…mehr
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences. Methods in Enzymology is now available online at ScienceDirect - full-text online of volumes 1 onwards. For more information about the Elsevier Book Series on ScienceDirect Program, please visit: http://www.info.sciencedirect.com/bookseries/This volume features methods for the study of globin and other nitric oxide-reactive proteins.
Professor Robert K Poole is Emeritus Professor of Microbiology at the University of Sheffield, UK. He was previously West Riding Professor of Microbiology at Sheffield and until 1996 held a Personal Chair in Microbiology at King's College London. During his long career, he has been awarded several research Fellowships, and taken sabbatical leave at the Australian National University, Kyoto University and Cornell University. His career-long interests have been in the areas of bacterial respiratory metabolism, metal-microbe interactions and bioactive small gas molecules. In particular, he has made notable contributions to bacterial terminal oxidases and resistance to nitric oxide with implications for bacterial pathogenesis. He co-discovered the flavohaemoglobin Hmp, now recognised as the preeminent mechanism of nitric oxide resistance in bacteria. He has served as Chairman of numerous research council grant committees, held research grants for over 40 years and published extensively (h-index, 2024 = 70). He served on several Institute review panels in the UK and overseas. He is a Fellow of the Royal Society of Chemistry and the Royal Society of Biology.
Inhaltsangabe
Section I. Nitric Oxide: Chemical and Analytical Methods
Chapter 1 The Chemistry of Nitric Oxide and Related Species Martin H. Hughes
Chapter 2 Delivery of nitric oxide for analysis of the function of cytochrome c'. Lindsay J. Cole, Wilhelmina M. Huston, and James W.B. Moir
Chapter 3 The preparation and purification of NO gas and the use of NO releasers. The application of NO donors and other agents of nitrosative stress in biological systems Rubina G. Aga and Martin N. Hughes
Chapter 4 The Chemistry of Peroxynitrite - Implications for Biological Activity Sara Goldstein and Gabor Merényi
Chapter 5 Nitric Oxide (NO) Selective Electrodes Ian R. Davies, Xueji Zhang
Chapter 6 NO, N2O and O2 Reaction Kinetics: Scope and Limitations of the Clark Electrode L.A.M. Pouvreau, M. J. F. Strampraad, S. Van Berloo, J. H. Kattenberg and S. de Vries
Chapter 7 Chemiluminescence quantification of Nitric oxide and its derivatives in liquid samples Jay R. Laver, Tânia M. Stevanin, Robert C. Read
Section II. Bacterial and Archaeal Hemoglobins
Chapter 8 Interactions of nitric oxide with hemoglobin: from microbes to man Michael Angelo, Alfred Hausladen and Jonathan S. Stamler
Chapter 9 Expression and purification of E. coli Hmp Robert K. Poole
Chapter 10 Structural studies on flavohemoglobins Andrea Ilari and Alberto Boffi
Chapter 11 Flavohemoglobin of Staphylococcus aureus Lígia S. Nobre, Vera L. Gonçalves and Lígia M. Saraiva
Chapter 12 Assay and Characterization of the Nitric Oxide Dioxygenase Activity of (Flavo)Hemoglobins Paul R. Gardner
Chapter 13 Globin Interactions with Lipids and Membranes Antonio Di Giulio and Alessandra Bonamore
Chapter 14 Assessment of Biotechnologically Relevant Characteristics of Heterologous Hemoglobins in Escherichia coli Pauli T. Kallio, Christian J. T. Bollinger, Taija Koskenkorva, and Alexander D. Frey
Chapter 15 Applications of the Vitreoscilla hemoglobin (VHb) gene (vgb) for improved microbial fermentation processes Xiao-Xing WEI, Guo-Qiang CHEN
Chapter 16 Expression and Purification of Cgb and Ctb, the NO-Inducible Globins of the Foodborne Bacterial Pathogen Campylobacter jejuni James L. Pickford, Laura Wainwright, Guanghui Wu, Robert K. Poole
Chapter 17 Mapping Heme-Ligand Tunnel in Group I Truncated(2/2) Hemoglobins Alessandra Pesce, Mario Milani, Marco Nardini and Martino Bolognesi
Chapter 18 Scavenging of reactive nitrogen species by mycobacterial truncated hemoglobins Paolo Ascenzi and Paolo Visca
Section III. Other Hemoglobins
Chapter 19 Expression, purification and crystallisation of neuro-and cytoglobin Sylvia Dewilde, Kirsten Mees, Laurent Coger, Christophe Lechauve, Michael C. Marden, Alexandra Pesce, Martino Bolognesi, Luc Monees
Chapter 20 Measurement of distal histidine coordination equilibrium and kinetics in hexacoordinate hemoglobins. Benoit J. Smagghe, Puspita Halder, and Mark S. Hargrove
Chapter 21 Purification of Class 1 Plant Hemoglobins and Examination of their Functional Properties Abir U. Igamberdiev and Robert D. Hill
Chapter 22 Use of in silico (computer) methods to predict and analyze the tertiary structure of plant hemoglobins Sabarinathan Kuttalingam Gopalasubramaniam, Verónica Garrocho-Villegas, Genoveva Bustos Rivera, Nina Pastor and Raúl Arredondo-Peter
Chapter 23 A Self-induction Method to Produce High Quantities of Recombinant Functional Flavo-Leghemoglobin Reductase. Estibaliz Urarte, Iñigo Auzmendi, Selene Rol, Idoia Ariz, Pedro Aparicio-Tejo, Raúl Arredondo-Peter and Jose F. Moran
Chapter 24 Spectroscopic and crystallographic characterization of bis-histidyl adducts in tetrameric hemoglobins Alessandro Vergara, Luigi Vitagliano, Cinzia Verde, Gu
Section I. Nitric Oxide: Chemical and Analytical Methods
Chapter 1 The Chemistry of Nitric Oxide and Related Species Martin H. Hughes
Chapter 2 Delivery of nitric oxide for analysis of the function of cytochrome c'. Lindsay J. Cole, Wilhelmina M. Huston, and James W.B. Moir
Chapter 3 The preparation and purification of NO gas and the use of NO releasers. The application of NO donors and other agents of nitrosative stress in biological systems Rubina G. Aga and Martin N. Hughes
Chapter 4 The Chemistry of Peroxynitrite - Implications for Biological Activity Sara Goldstein and Gabor Merényi
Chapter 5 Nitric Oxide (NO) Selective Electrodes Ian R. Davies, Xueji Zhang
Chapter 6 NO, N2O and O2 Reaction Kinetics: Scope and Limitations of the Clark Electrode L.A.M. Pouvreau, M. J. F. Strampraad, S. Van Berloo, J. H. Kattenberg and S. de Vries
Chapter 7 Chemiluminescence quantification of Nitric oxide and its derivatives in liquid samples Jay R. Laver, Tânia M. Stevanin, Robert C. Read
Section II. Bacterial and Archaeal Hemoglobins
Chapter 8 Interactions of nitric oxide with hemoglobin: from microbes to man Michael Angelo, Alfred Hausladen and Jonathan S. Stamler
Chapter 9 Expression and purification of E. coli Hmp Robert K. Poole
Chapter 10 Structural studies on flavohemoglobins Andrea Ilari and Alberto Boffi
Chapter 11 Flavohemoglobin of Staphylococcus aureus Lígia S. Nobre, Vera L. Gonçalves and Lígia M. Saraiva
Chapter 12 Assay and Characterization of the Nitric Oxide Dioxygenase Activity of (Flavo)Hemoglobins Paul R. Gardner
Chapter 13 Globin Interactions with Lipids and Membranes Antonio Di Giulio and Alessandra Bonamore
Chapter 14 Assessment of Biotechnologically Relevant Characteristics of Heterologous Hemoglobins in Escherichia coli Pauli T. Kallio, Christian J. T. Bollinger, Taija Koskenkorva, and Alexander D. Frey
Chapter 15 Applications of the Vitreoscilla hemoglobin (VHb) gene (vgb) for improved microbial fermentation processes Xiao-Xing WEI, Guo-Qiang CHEN
Chapter 16 Expression and Purification of Cgb and Ctb, the NO-Inducible Globins of the Foodborne Bacterial Pathogen Campylobacter jejuni James L. Pickford, Laura Wainwright, Guanghui Wu, Robert K. Poole
Chapter 17 Mapping Heme-Ligand Tunnel in Group I Truncated(2/2) Hemoglobins Alessandra Pesce, Mario Milani, Marco Nardini and Martino Bolognesi
Chapter 18 Scavenging of reactive nitrogen species by mycobacterial truncated hemoglobins Paolo Ascenzi and Paolo Visca
Section III. Other Hemoglobins
Chapter 19 Expression, purification and crystallisation of neuro-and cytoglobin Sylvia Dewilde, Kirsten Mees, Laurent Coger, Christophe Lechauve, Michael C. Marden, Alexandra Pesce, Martino Bolognesi, Luc Monees
Chapter 20 Measurement of distal histidine coordination equilibrium and kinetics in hexacoordinate hemoglobins. Benoit J. Smagghe, Puspita Halder, and Mark S. Hargrove
Chapter 21 Purification of Class 1 Plant Hemoglobins and Examination of their Functional Properties Abir U. Igamberdiev and Robert D. Hill
Chapter 22 Use of in silico (computer) methods to predict and analyze the tertiary structure of plant hemoglobins Sabarinathan Kuttalingam Gopalasubramaniam, Verónica Garrocho-Villegas, Genoveva Bustos Rivera, Nina Pastor and Raúl Arredondo-Peter
Chapter 23 A Self-induction Method to Produce High Quantities of Recombinant Functional Flavo-Leghemoglobin Reductase. Estibaliz Urarte, Iñigo Auzmendi, Selene Rol, Idoia Ariz, Pedro Aparicio-Tejo, Raúl Arredondo-Peter and Jose F. Moran
Chapter 24 Spectroscopic and crystallographic characterization of bis-histidyl adducts in tetrameric hemoglobins Alessandro Vergara, Luigi Vitagliano, Cinzia Verde, Gu
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