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Hepatitis B is the most important infectious occupational disease for the dental health care workers (DHCWs). DHCWs frequently come in contact with blood and saliva and hence are at risk for Hepatitis B Virus (HBV) exposure. Studies indicate that DHCWs, through occupational exposure, may have a 10 times greater risk of becoming a chronic hepatitis B carrier than the average citizen. A cross sectional seroprevalence study was conducted which included dental students who were enrolled in the dental institution. Their blood samples were collected and tested by Enzyme Linked Immunosorbent assay (ELISA) for Hepatitis B surface antigen (HBsAg) and Anti-HBsAg antibody titres. Though a low rate of HBV infection was observed among dental students, a significant number of dental students were non-vaccinated which suggests a need for proactive implementation of HBV vaccination programme.