Intermediate Filament Proteins, the latest volume in the Methods in Enzymology series covers all the intermediate filaments in vertebrates and invertebrates, providing a unique understanding of the multiple different tissue-specific intermediate filaments. This volume also covers the latest methods that are currently being used to study intermediate filament protein function and dynamics. It will be an important companion for any experimentalist interesting in studying this protein family in their cell or organism model system.
Intermediate Filament Proteins, the latest volume in the Methods in Enzymology series covers all the intermediate filaments in vertebrates and invertebrates, providing a unique understanding of the multiple different tissue-specific intermediate filaments.
This volume also covers the latest methods that are currently being used to study intermediate filament protein function and dynamics. It will be an important companion for any experimentalist interesting in studying this protein family in their cell or organism model system.
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Inhaltsangabe
Part I: General Methods to Study IF Proteins 1. How to Study Intermediate Filaments in Atomic Detail Anastasia A. Chernyatina, John F. Hess, Dmytro Guzenko, John C. Voss and Sergei V. Strelkov 2. Mechanical Properties of Intermediate Filament Proteins Elisabeth E. Charrier and Paul A. Janmey 3. Multidimensional Monitoring of Keratin Intermediate Filaments in Cultured Cells and Tissues Nicole Schwarz, Marcin Moch, Reinhard Windoffer and Rudolf E. Leube 4. Phospho-Specific Antibody Probes of Intermediate Filament (IF) Proteins Hidemasa Goto, Hiroki Tanaka, Kousuke Kasahara and Masaki Inagaki 5. Assays for Post-Translational Modifications of Intermediate Filament Proteins Natasha T. Snider and M. Bishr Omary 6. Immunofluorescence and Immunohistochemical Detection of Keratins Cornelia Stumptner, Margit Gogg-Kamerer, Christian Viertler, Helmut Denk and Kurt Zatloukal 7. High Throughput Screening for Drugs that Modulate Intermediate Filament Proteins Jingyuan Sun , Vincent E. Groppi , Honglian Gui, Lu Chen, Qing Xie, Li Liu and M. Bishr Omary 8. The Use of Withaferin A to Study Intermediate Filaments Royce Mohan and Paola BargagnaMohan 9. Assays to Study Consequences of Cytoplasmic Intermediate Filament Mutations: the Case of Epidermal Keratins Tong San Tan, Yi Zhen Ng, Cedric Badowski, Tram Dang, John E A Common, Lukas Lacina, Ildikó Szeverényi and E Birgitte Lane 10. Using Data Mining and Computational Approaches to Study Intermediate Filament Structure and Function David A.D. Parry Part II: Mammalian IF Proteins 11. Isolation and Analysis of Keratins and Keratin Associated Proteins from Hair and Wool Santanu Deb-Choudhury, Jeffrey E. Plowman and Duane P. Harland 12. Skin Keratins Fengrong Wang, Abigail Zieman and Pierre A. Coulombe 13. Simple Epithelial Keratins Pavel Strnad, Nurdan Guldiken, Terhi O. Helenius, Julia O. Misiorek, Joel H. Nyström, Iris A.K. Lähdeniemi, Jonas S.G. Silvander, Deniz Kuscuoglu and Diana M. Toivola 14. Methods for Determining the Cellular Functions of Vimentin Intermediate Filaments Karen M. Ridge, Dale Shumaker, Amélie Robert, Caroline Hookway, Vladimir I. Gelfand, Paul A. Janmey, Jason Lowery, Ming Guo, David A. Weitz, Edward Kuczmarski, and Robert D. Goldman 15. Strategies to Study Desmin in Cardiac Muscle and Culture Systems Antigoni Diokmetzidou, Mary Tsikitis, Sofia Nikouli, Ismini Kloukina, Elsa Tsoupri, Stamatis Papathanasiou, Stelios Psarras, Manolis Mavroidis and Yassemi Capetanaki 16. Genetic Manipulation of Neurofilament Protein Phosphorylation Maria R. Jones, Eric Villalón and Michael L. Garcia 17. ?-Internexin and Peripherin: Expression, Assembly, Functions and Roles in Disease Jian Zhao and Ronald K. H. Liem 18. Studying Nestin and its Inter-Relationship with Cdk5 Julia Lindqvist, Num Wistbacka and John E. Eriksson 19. Synemin: Molecular Features and the Use of Proximity Ligation Assay to Study its Interactions Madhumita Paul and Omar Skalli 20. Targeting Mitogen-Activated Protein Kinase Signaling in Mouse Models of Cardiomyopathy Caused By Lamin A/C Gene Mutations Antoine Muchir and Howard J Worman 21. In Vivo, Ex Vivo and in Vitro Approaches to Study Intermediate Filaments in the Eye Lens M. Jarrin, L. Young, W. Wu, J.M. Girkin and R.A. Quinlan Part III: Non-Mammalian IF Protein Systems 22. Compartment Specific Phosphorylation of Squid Neurofilaments Philip Grant and Harish C. Pant 23. Using Xenopus Embryos to Study Transcriptional and Post-Transcriptional Gene Regulatory Mechanisms of Intermediate Filaments Chen Wang and Ben G. Szaro 24. Intermediate Filaments in Caenorhabditis Elegans Noam Zuela and Yosef Gruenbaum 25. Mechanical Probing of the Intermediate Filament-Rich C. Elegans Intestine Oliver Jahnel, Bernd Hoffmann, Rudolf Merkel, Olaf Bossinger and Rudolf E. Leube 26. Using Drosophila for Studies of Intermedia
Part I: General Methods to Study IF Proteins 1. How to Study Intermediate Filaments in Atomic Detail Anastasia A. Chernyatina, John F. Hess, Dmytro Guzenko, John C. Voss and Sergei V. Strelkov 2. Mechanical Properties of Intermediate Filament Proteins Elisabeth E. Charrier and Paul A. Janmey 3. Multidimensional Monitoring of Keratin Intermediate Filaments in Cultured Cells and Tissues Nicole Schwarz, Marcin Moch, Reinhard Windoffer and Rudolf E. Leube 4. Phospho-Specific Antibody Probes of Intermediate Filament (IF) Proteins Hidemasa Goto, Hiroki Tanaka, Kousuke Kasahara and Masaki Inagaki 5. Assays for Post-Translational Modifications of Intermediate Filament Proteins Natasha T. Snider and M. Bishr Omary 6. Immunofluorescence and Immunohistochemical Detection of Keratins Cornelia Stumptner, Margit Gogg-Kamerer, Christian Viertler, Helmut Denk and Kurt Zatloukal 7. High Throughput Screening for Drugs that Modulate Intermediate Filament Proteins Jingyuan Sun , Vincent E. Groppi , Honglian Gui, Lu Chen, Qing Xie, Li Liu and M. Bishr Omary 8. The Use of Withaferin A to Study Intermediate Filaments Royce Mohan and Paola BargagnaMohan 9. Assays to Study Consequences of Cytoplasmic Intermediate Filament Mutations: the Case of Epidermal Keratins Tong San Tan, Yi Zhen Ng, Cedric Badowski, Tram Dang, John E A Common, Lukas Lacina, Ildikó Szeverényi and E Birgitte Lane 10. Using Data Mining and Computational Approaches to Study Intermediate Filament Structure and Function David A.D. Parry Part II: Mammalian IF Proteins 11. Isolation and Analysis of Keratins and Keratin Associated Proteins from Hair and Wool Santanu Deb-Choudhury, Jeffrey E. Plowman and Duane P. Harland 12. Skin Keratins Fengrong Wang, Abigail Zieman and Pierre A. Coulombe 13. Simple Epithelial Keratins Pavel Strnad, Nurdan Guldiken, Terhi O. Helenius, Julia O. Misiorek, Joel H. Nyström, Iris A.K. Lähdeniemi, Jonas S.G. Silvander, Deniz Kuscuoglu and Diana M. Toivola 14. Methods for Determining the Cellular Functions of Vimentin Intermediate Filaments Karen M. Ridge, Dale Shumaker, Amélie Robert, Caroline Hookway, Vladimir I. Gelfand, Paul A. Janmey, Jason Lowery, Ming Guo, David A. Weitz, Edward Kuczmarski, and Robert D. Goldman 15. Strategies to Study Desmin in Cardiac Muscle and Culture Systems Antigoni Diokmetzidou, Mary Tsikitis, Sofia Nikouli, Ismini Kloukina, Elsa Tsoupri, Stamatis Papathanasiou, Stelios Psarras, Manolis Mavroidis and Yassemi Capetanaki 16. Genetic Manipulation of Neurofilament Protein Phosphorylation Maria R. Jones, Eric Villalón and Michael L. Garcia 17. ?-Internexin and Peripherin: Expression, Assembly, Functions and Roles in Disease Jian Zhao and Ronald K. H. Liem 18. Studying Nestin and its Inter-Relationship with Cdk5 Julia Lindqvist, Num Wistbacka and John E. Eriksson 19. Synemin: Molecular Features and the Use of Proximity Ligation Assay to Study its Interactions Madhumita Paul and Omar Skalli 20. Targeting Mitogen-Activated Protein Kinase Signaling in Mouse Models of Cardiomyopathy Caused By Lamin A/C Gene Mutations Antoine Muchir and Howard J Worman 21. In Vivo, Ex Vivo and in Vitro Approaches to Study Intermediate Filaments in the Eye Lens M. Jarrin, L. Young, W. Wu, J.M. Girkin and R.A. Quinlan Part III: Non-Mammalian IF Protein Systems 22. Compartment Specific Phosphorylation of Squid Neurofilaments Philip Grant and Harish C. Pant 23. Using Xenopus Embryos to Study Transcriptional and Post-Transcriptional Gene Regulatory Mechanisms of Intermediate Filaments Chen Wang and Ben G. Szaro 24. Intermediate Filaments in Caenorhabditis Elegans Noam Zuela and Yosef Gruenbaum 25. Mechanical Probing of the Intermediate Filament-Rich C. Elegans Intestine Oliver Jahnel, Bernd Hoffmann, Rudolf Merkel, Olaf Bossinger and Rudolf E. Leube 26. Using Drosophila for Studies of Intermedia
Rezensionen
Praise for the Series: "Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry "The work most often consulted in the lab." --Enzymologia "The Methods in Enzymology series represents the gold-standard." --Neuroscience
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