Laboratory Methods in Enzymology: Protein Part B

Herausgegeben:Lorsch, Jon

• Gebundenes Buch

Produktbeschreibung

Laboratory Methods in Enzymology: Protein Part B brings together a number of core protocols concentrating on protein, carefully written and edited by experts.

Produktdetails

• Methods in Enzymology Volume 539
• Verlag: Academic Press / Elsevier Science & Technology
• Artikelnr. des Verlages: C2013-0-09947-1
• Englisch
• Abmessung: 14mm x 152mm x 229mm
• Gewicht: 494g
• ISBN-13: 9780124201200
• Artikelnr.: 40821371

Inhaltsangabe

Small-scale expression of proteins in E. coli
Protein expression-Yeast
Recombinant protein expression in baculovirus-infected insect cells
Single cell cloning of a stable mammalian cell line
In vitro synthesis of proteins in bacterial extracts
Preparation of a Saccharomyces cerevisiae cell-free extract for in vitro translation
Yeast two-hybrid screen
UV cross-linking of interacting RNA and protein in cultured cells
Analysis of RNA-protein interactions by cell mixing
General protein-protein cross-linking
Chromatin immunoprecipitation and multiplex sequencing (ChIP-Seq) to identify global transcription factor binding sites in the nematode Caenorhabditis elegans
PAR-CLIP (photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation): A step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins
Determining the RNA specificity and targets of RNA-binding proteins using a three-hybrid system
Dissecting a known RNA-protein interaction using a yeast three-hybrid system
Identifying proteins that bind a known RNA sequence using the yeast three-hybrid system