Laser Capture Microdissection

Methods and Protocols
Herausgegeben:Noberini, Roberta

• Gebundenes Buch

Produktbeschreibung

This volume details protocols that highlight the versatility of LCM across different biological contexts and analytical workflows. Chapters cover protocols on isolating cells from blood smears, live cell samples, and FFPE or frozen tissues. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and practical, Laser Capture Microdissection: Methods and Protocols aims to support both new and experienced researchers in effectively applying LCM to their own biological questions, across a wide variety of systems and technical platforms.

Produktdetails

• Methods in Molecular Biology 3015
• Verlag: Springer, Berlin; Humana
• Seitenzahl: 190
• Erscheinungstermin: 24. März 2026
• Englisch
• ISBN-13: 9781071651537
• ISBN-10: 1071651536
• Artikelnr.: 75863436

Inhaltsangabe

Laser capture microdissection from blood smears.
 Live cell isolation by laser capture microdissection.
From Laser Microdissection to Spatial Glycomics: Lectin Microarray Protocols for Tissue Glycome Mapping with High
End Scanners.
Laser capture microdissection followed by DNA methylation profiling.
High resolution laser microdissection to investigate transcriptional states in formalin
fixed paraffin embedded samples.
Transcriptomic analysis from cryosectioned tissue samples using laser capture microdissection based Geo
seq.
Immunofluorescence imaging
guided laser microdissection for ultra
low input spatial tissue proteomics.
 Low
Input Proteomics Protocols for Laser
Captured FFPE Samples with Orbitrap Astral DIA
MS.
Laser capture microdissection followed by histone H1 variant analysis by mass spectrometry.
 A spatial proteomics approach to isolate and analyze amyloid proteins using laser capture microdissection and mass spectrometry.
 Chromatin Immunoprecipitation
Sequencing and RNA
Sequencing from Laser Microdissected Human Lung Cancer Cells.
 Laser capture microdissection followed by ATAC
seq.
 Laser Capture Microdissection for quantitative shotgun lipidomics.
 Artificial Intelligence
driven laser capture microdissection.
 Automated Intelligent Microscopy for phenotypic identification, spatial localization and retargeting of cells.