Lipid-Protein Interactions

Methods and Protocols
Herausgegeben:Kleinschmidt, Jörg H.

• Gebundenes Buch

Produktbeschreibung

This third edition expands upon the chapters of the previous editions, providing new and updated methods. Chapters explore protein-lipid interactions, membranes, membrane protein structure, how membrane proteins affect lipids and how they are in turn affected by the lipid bilayer, and lipid properties. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Lipid-Protein Interactions: Methods and Protocols, Third Edition is an essential resource for all researchers who are interested in obtaining up-to-date and comprehensive information about membrane structure and function.

Produktdetails

• Methods in Molecular Biology 3001
• Verlag: Springer, Berlin; Humana
• Artikelnr. des Verlages: 89279991
• 3. Aufl. 2026.
• Seitenzahl: 788
• Erscheinungstermin: 23. Januar 2026
• Englisch
• Abmessung: 254mm x 178mm
• ISBN-13: 9781071650530
• ISBN-10: 107165053X
• Artikelnr.: 75410769

Inhaltsangabe

Characterization of membrane binding and protein
lipid interactions at atomic level with accelerated HMMM model.
Quartz Crystal Microbalances as Tools for Probing Protein
Membrane Interactions.
Surface Plasmon Resonance for Measuring Interactions of Proteins with Lipids and Lipid Membranes.
Isothermal Titration Calorimetry for Investigating Thermodynamics of Protein
Lipid Interactions.
Differential Scanning Calorimetry of Protein
Lipid Interactions.
Purification of Bacterial Lipopolysaccharide, and how to study its interactions with proteins.
Analysis of the Kinetics of Folding and Insertion of β
Barrel Membrane Proteins by Gel
Electrophoresis.
Imaging Lipid
Related Macromolecular Structures Using Optimized Negative Staining Transmission Electron Microscopy.
Probing the Annular Lipid belt of Membrane Proteins Using Native Mass Spectrometry.
Protein Microarrays and Liposome: A Method for Studying Lipid
Protein Interactions.
Complex Structural Examination of Protein
Lipid Interactions with Neutron Scattering Techniques.
Circular
Dichroism and Synchrotron
Radiation Circular
Dichroism Spectroscopy as Tools to Monitor Protein Structure in a Lipid Environment.
Protein
Membrane Interactions Studied by Transmission and Total Internal Reflection FTIR Spectroscopy.
Deep UV Resonance Raman Spectroscopy: Applications to Membrane Proteins and Peptides.
Analyzing Transmembrane Protein and Hydrophobic Helix Topography by Dual Fluorescence Quenching.
Förster Resonance Energy Transfer as a Tool for Quantification of Protein
Lipid Selectivity.
A Guide to Tracking Single Membrane Proteins and Their Interactions in Supported Lipid Bilayers.
Fluorescence Correlation Spectroscopy to Examine Protein
Lipid Interactions in Membranes.
Membrane Pore Formation by Peptides Studied by Fluorescence Techniques.
Sited
directed fluorescence spectroscopy for studies on the folding and insertion of proteins in lipid bilayers and model membranes.
EPR Techniques to Probe Insertion and Conformation of Spin
Labeled Proteins in Lipid Bilayers.
Electron paramagnetic resonance spectroscopy of spin
labeled lipids for studying lipid
protein interaction in model
and biomembranes.
Non
linear continuous wave electron paramagnetic resonance spectroscopy for investigation of lipid
protein interactions in membranes.