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A rapid, sensitive, and specific RP-HPLC method with UV detection was developed and validated for the determination of voriconazole. Chromatography was performed on an Enable C18 G column (250 × 4.6 mm) in isocratic mode, using a mobile phase of methanol and acetonitrile (60:40, v/v). The flow rate was set at 1 mL/min, and the effluent was monitored at 223 nm. Chromatographic analysis revealed a main peak for voriconazole at a retention time of 2.841 minutes. Methanol was selected as the solvent for the analysis, with the max determined to be 223 nm. The method exhibited linearity over a concentration range of 5-30 µg/mL. Validation parameters, including linearity, range, precision, accuracy, limit of detection (LOD), and limit of quantitation (LOQ), were assessed and met the required standards.