Going beyond step-by-step descriptions of how to perform techniques, Methods in Neuroendocrinology examines the questions being asked in the field, including what problems need to be solved and what the limits of interpretation are. The experimental approaches described in this book merge endocrine and neuroscientific research and offer insight into the many disciplines involved. Much more than a "cookbook", this is a resource for every researcher who wants to pursue new areas of inquiry in the science of neuroendocrinology.
Going beyond step-by-step descriptions of how to perform techniques, Methods in Neuroendocrinology examines the questions being asked in the field, including what problems need to be solved and what the limits of interpretation are. The experimental approaches described in this book merge endocrine and neuroscientific research and offer insight into the many disciplines involved. Much more than a "cookbook", this is a resource for every researcher who wants to pursue new areas of inquiry in the science of neuroendocrinology.
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Inhaltsangabe
Summary: (1) What are the cellular and molecular mechanisms responsible for the synthesis and secretion of hormones? (2) How do other cells influence neuroendocrine control mechanisms? (3) How do hormones signal their neuroendocrine regulatory neurons? (4) How can we use hormones as indicators of specific changes in neuroendocrine control sites in the brain? Studying morphology of the immortalized hypothalamic LHRH neurons.- Assessment of neuropeptide receptor gene expression using reverse transcriptase polymerase chain reaction.- Identification of biotinylated ligands on specific target cells in the pituitary.- Steroid hormone receptors and the assessment of feedback sensitivity.- In vitro approaches to neuroendocrinology.- Intracellular recording from hypothalamic cells that regulate neuroendocrine function.- Application of in vivo electrophysiological approaches to the study of neuroendocrine systems.- Antisense oligonucleotides.
Chapter 1 Functional Morphology of the Immortalized Hypothalamic LHRH Neurons/William C. Wetsel, Istvdn Merchenthaler, and Zsolt Liposits Chapter 2 Assessment of Neuropeptide Receptor Gene Expression Using Reverse Transcriptase Polymerase Chain Reaction (RT PCR)/Janice H. Urban Chapter 3 Identification of Biotinylated Ligands on Specific Target Cells in the Pituitary Studies of Regulation of Binding/Gwendolyn V Childs Chapter 4 Steroid Hormone Receptors and the Assessment of Feedback Sensitivity/Robert J. Handaf Richard H. Price Jr.t and Melinda E. Wilson Chapter 5 In Vitro Approaches to Neuroendocrinology/Celia D. Sladek Chapter 6 Intracellular Recording from Hypothalamic Cells that Regulate Neuroendocrine Function/Bret N. Smith and F. Edward Dudek Chapter 7 Application of In Vivo Electrophysiological Approaches to the Study of Neuroendocrine Systems/Joan M. Lakoski and Jane E. Smith Chapter 8 Antisense Oligonucleotides as Tools in Neuroendocrinology/Mariana Morris and PingLi Chapter 9 The Role of Biogenic Amines in Neuroendocrine Regulation in ConsciousRats/Gydrgy Bagdy Chapter 10 Neuroendocrinology of Stress: Behavioral and Neurobiological Considerations/Bela G.J. Bohus Chapter 11 Issues in Quantifying Pulsatile Neurohormone Release Johannes D. Veldhuis Chapter 12 Neuroendocrine Challenge Tests: What Can We Learn From Them ?/Philip J. Cowen Index.
Summary: (1) What are the cellular and molecular mechanisms responsible for the synthesis and secretion of hormones? (2) How do other cells influence neuroendocrine control mechanisms? (3) How do hormones signal their neuroendocrine regulatory neurons? (4) How can we use hormones as indicators of specific changes in neuroendocrine control sites in the brain? Studying morphology of the immortalized hypothalamic LHRH neurons.- Assessment of neuropeptide receptor gene expression using reverse transcriptase polymerase chain reaction.- Identification of biotinylated ligands on specific target cells in the pituitary.- Steroid hormone receptors and the assessment of feedback sensitivity.- In vitro approaches to neuroendocrinology.- Intracellular recording from hypothalamic cells that regulate neuroendocrine function.- Application of in vivo electrophysiological approaches to the study of neuroendocrine systems.- Antisense oligonucleotides.
Chapter 1 Functional Morphology of the Immortalized Hypothalamic LHRH Neurons/William C. Wetsel, Istvdn Merchenthaler, and Zsolt Liposits Chapter 2 Assessment of Neuropeptide Receptor Gene Expression Using Reverse Transcriptase Polymerase Chain Reaction (RT PCR)/Janice H. Urban Chapter 3 Identification of Biotinylated Ligands on Specific Target Cells in the Pituitary Studies of Regulation of Binding/Gwendolyn V Childs Chapter 4 Steroid Hormone Receptors and the Assessment of Feedback Sensitivity/Robert J. Handaf Richard H. Price Jr.t and Melinda E. Wilson Chapter 5 In Vitro Approaches to Neuroendocrinology/Celia D. Sladek Chapter 6 Intracellular Recording from Hypothalamic Cells that Regulate Neuroendocrine Function/Bret N. Smith and F. Edward Dudek Chapter 7 Application of In Vivo Electrophysiological Approaches to the Study of Neuroendocrine Systems/Joan M. Lakoski and Jane E. Smith Chapter 8 Antisense Oligonucleotides as Tools in Neuroendocrinology/Mariana Morris and PingLi Chapter 9 The Role of Biogenic Amines in Neuroendocrine Regulation in ConsciousRats/Gydrgy Bagdy Chapter 10 Neuroendocrinology of Stress: Behavioral and Neurobiological Considerations/Bela G.J. Bohus Chapter 11 Issues in Quantifying Pulsatile Neurohormone Release Johannes D. Veldhuis Chapter 12 Neuroendocrine Challenge Tests: What Can We Learn From Them ?/Philip J. Cowen Index.
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