This book is a must-have for anyone interested in obesity or the physiology of white or brown adipose tissues. It contains state-of-the-art methods from researchers that are world leaders in this field. Detailed lab protocols range from methods to visualize adipocytes and adipose tissues in humans and experimental models, to convert stem cells into white and brown adipocytes in vitro, to evaluate aspects of adipocyte metabolism, to inducibly knock out genes in adipose tissues, and to evaluate transcriptional control of adipogenesis on a global scale.
This book is a must-have for anyone interested in obesity or the physiology of white or brown adipose tissues. It contains state-of-the-art methods from researchers that are world leaders in this field. Detailed lab protocols range from methods to visualize adipocytes and adipose tissues in humans and experimental models, to convert stem cells into white and brown adipocytes in vitro, to evaluate aspects of adipocyte metabolism, to inducibly knock out genes in adipose tissues, and to evaluate transcriptional control of adipogenesis on a global scale.
1. Preparation and Differentiation of Mesenchymal Stem Cells from Ears of Adult Mice 2. 3-D Adipocyte Differentiation and Peri-Adipocyte Collagen Turnover 3. Differentiation of White and Brown Adipocytes from Human Pluripotent Stem Cells 4. Optimal Protocol for the Differentiation and Metabolic Analysis of Human Adipose Stromal Cells 5. Human Adipose Derived Stromal/Stem Cell Isolation, Culture and Osteogenic Differentiation 6. Analysis of Adipose Tissue Lipid Using Mass Spectrometry 7. Measurement of Long-Chain Fatty Acid Uptake into Adipocytes 8. Measurement of the Unfolded Protein Response (UPR) to Investigate its Role in Adipogenesis and Obesity 9. Application of Activity-Based Protein Profiling to Study Enzyme Function in Adipocytes 10. Measurement of Lipolysis 11. Measurement of Lipolysis Products Secreted by 3t3-L1 Adipocytes Using Microfluidics 12. Methods for Performing Lipidomics in White Adipose Tissue 13. Measuring Respiratory Activity of Adipocytes and Adipose Tissues in Real Time 14. Detecting Protein Carbonylation in Adipose Tissue and in Cultured Adipocytes 15. Use of Fluorescence Microscopy to Probe Intracellular Lipolysis 16. Evaluation of Protein Phosphorylation during Adipogenesis
1. Preparation and Differentiation of Mesenchymal Stem Cells from Ears of Adult Mice 2. 3-D Adipocyte Differentiation and Peri-Adipocyte Collagen Turnover 3. Differentiation of White and Brown Adipocytes from Human Pluripotent Stem Cells 4. Optimal Protocol for the Differentiation and Metabolic Analysis of Human Adipose Stromal Cells 5. Human Adipose Derived Stromal/Stem Cell Isolation, Culture and Osteogenic Differentiation 6. Analysis of Adipose Tissue Lipid Using Mass Spectrometry 7. Measurement of Long-Chain Fatty Acid Uptake into Adipocytes 8. Measurement of the Unfolded Protein Response (UPR) to Investigate its Role in Adipogenesis and Obesity 9. Application of Activity-Based Protein Profiling to Study Enzyme Function in Adipocytes 10. Measurement of Lipolysis 11. Measurement of Lipolysis Products Secreted by 3t3-L1 Adipocytes Using Microfluidics 12. Methods for Performing Lipidomics in White Adipose Tissue 13. Measuring Respiratory Activity of Adipocytes and Adipose Tissues in Real Time 14. Detecting Protein Carbonylation in Adipose Tissue and in Cultured Adipocytes 15. Use of Fluorescence Microscopy to Probe Intracellular Lipolysis 16. Evaluation of Protein Phosphorylation during Adipogenesis
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