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This research determined the rate and extent of aerobic biodegradation of fuel oxygenates ethyl tert butyl ether (ETBE), tert amyl methyl ether (TAME), and ethyl alcohol (ethanol). Biodegradation was measured using gas chromatography (GC), respirometry, and biochemical oxygen demand (BOD) tests. Additionally, the research determined the effects of toluene on degradation rates. This microcosm study used a microbial consortium obtained from a petroleum refinery wastewater treatment facility. Respirometry data were collected from chambers containing pure oxygenates, or oxygenate/toluene mixtures. Samples were withdrawn periodically for GC analysis. Aerobic conditions were maintained in the chambers at all times. The five-day BOD test was conducted separately using Standard Methods. Degradation of oxygenates was compared to degradation of toluene, assuming first order decay. Across all experiments TAME degraded at 8.57% the rate of toluene. Similarly, ETBE degraded at 7.86% the rate of toluene. Ethanol was significantly faster, degrading at 158.26% the rate of toluene. GC and respirometry were the most suitable methods for measuring degradation. The BOD5 test provided acceptable results for toluene and ethanol, but not for the slower degrading oxygenates. Finally, the presence of toluene slowed the degradation of both ETBE and TAME. This work has been selected by scholars as being culturally important, and is part of the knowledge base of civilization as we know it. This work was reproduced from the original artifact, and remains as true to the original work as possible. Therefore, you will see the original copyright references, library stamps (as most of these works have been housed in our most important libraries around the world), and other notations in the work. This work is in the public domain in the United States of America, and possibly other nations. Within the United States, you may freely copy and distribute this work, as no entity (individual or corporate) has a copyright on the body of the work. As a reproduction of a historical artifact, this work may contain missing or blurred pages, poor pictures, errant marks, etc. Scholars believe, and we concur, that this work is important enough to be preserved, reproduced, and made generally available to the public. We appreciate your support of the preservation process, and thank you for being an important part of keeping this knowledge alive and relevant.