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Enzymatic conversion of glucose to fructose by glucose isomerase (GI) received considerable attention due to the most successful application of this enzyme in high fructose corn syrup (HFCS) production. HFCS could be used as a replacer for sucrose in beverages, baking, canning and confectionary industries. Glucose isomerase is an intracellular enzyme that produced by various microorganisms. To improve any fermentation process economy, different protocols should be considered such as: optimization of growth condition, mutation, protoplast fusion and immobilization. This book provides information about the microbial sources of GI, the techniques used in production improvement. Also, it deals with the purification and immobilization of this enzyme to be applicable in HFCS production. This book could help the biotechnologist and scientists who are working in the enzymes and genetic engineering field. It could be beneficial for anyone work in food industries who considering the utilization of HFCS.