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The geographical distribution of positive diagnosed mutations of thalassaemic patients observe in the map of Iraq, the results revealed the heterogeneity of diagnosed mutations within the Iraqi regions. As can be seen from the distribution of mutations presenting on the Iraqi map, IVS- -5 and codon 15 were more occurred in the middle and south of Iraq, whereas codon 8/9 and codon 8 mutations were more obvious in the middle and north of Iraq. In addition, -88 mutation was found in the nearby cities of western of Iraq.