Sue Carson (North Carolina State University, Raleigh, U.S.A.), Heather B. Miller (Department of Chemistry, High Point University,, Melissa C. Srougi (High Point Assistant Professor of Biochemistry
Molecular Biology Techniques
A Classroom Laboratory Manual
Sue Carson (North Carolina State University, Raleigh, U.S.A.), Heather B. Miller (Department of Chemistry, High Point University,, Melissa C. Srougi (High Point Assistant Professor of Biochemistry
Molecular Biology Techniques
A Classroom Laboratory Manual
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Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology-or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students will gain hands-on experience on subcloning a gene into an expression vector…mehr
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Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology-or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students will gain hands-on experience on subcloning a gene into an expression vector straight through to the purification of the recombinant protein.
Produktdetails
- Produktdetails
- Verlag: Elsevier Science Publishing Co Inc
- 4 ed
- Seitenzahl: 294
- Erscheinungstermin: 11. März 2019
- Englisch
- Abmessung: 273mm x 211mm x 16mm
- Gewicht: 832g
- ISBN-13: 9780128180242
- ISBN-10: 0128180242
- Artikelnr.: 54589524
- Herstellerkennzeichnung
- Libri GmbH
- Europaallee 1
- 36244 Bad Hersfeld
- gpsr@libri.de
- Verlag: Elsevier Science Publishing Co Inc
- 4 ed
- Seitenzahl: 294
- Erscheinungstermin: 11. März 2019
- Englisch
- Abmessung: 273mm x 211mm x 16mm
- Gewicht: 832g
- ISBN-13: 9780128180242
- ISBN-10: 0128180242
- Artikelnr.: 54589524
- Herstellerkennzeichnung
- Libri GmbH
- Europaallee 1
- 36244 Bad Hersfeld
- gpsr@libri.de
Dr. Susan Carson is a Professor of Plant and Microbial Biology at North Carolina State University (Raleigh, NC) where she has served on the faculty since 2001. She directs the Master of Microbial Biotechnology Program and also leads a uni?versity-wide faculty development program focused on enhancing students' critical and creative thinking skills across disci?plines. Prior to her current role, Dr. Carson spent over a decade leading curriculum development for the North Carolina State Biotechnology Program and two years as a Program Officer at the National Science Foundation Division of Undergraduate Education. Her current work focuses on college-level biology education, enhancing students' higher order thinking across disciplines, and integration of Design Thinking in a Professional Science Masters (PSM) program. She graduated from Rutgers University (New Brunswick, NJ) with a BS in Biotechnology, and from the University of North Carolina (Chapel Hill, NC) with a PhD in Microbiology.
Part I: Manipulation of DNAAdvanced Alternatives Within Part I 1. Getting
Oriented; Practicing With Micropipettes 2. Purification and Digestion of
Plasmid (Vector) DNA3. Completion of Vector Preparation and Polymerase
Chain Reaction Amplification of egfp 4. Preparation of Insert DNA (egfp)
PCR Product5. DNA Ligation and Transformation of Escherichia coli
Part II: Screening TransformantsAdvanced Alternatives Within Part II6.
Screening of Transformants, Part I 7. Screening of Transformants, Part II8.
Analysis of DNA Sequence From a Positive Clone, Part II
Part III: Expression, Detection, and Purification of Recombinant Proteins
from BacteriaAdvanced Alternatives Within Part III 9. Expression of Fusion
Protein from Positive Clones, SDS_PAGE and Western Blot: Part I 10.
Expression of Fusion Protein from Positive Clones, SDS_PAGE, and Western
Blot: Part II 11. Extraction of Recombinant Protein From Escherichia coli
Using a Glutathione Affinity Column12. Analysis of Purification Fractions
Part IV: Analysis of mRNA Levels13. Total RNA Purification14. Analysis of
gst::egfp mRNA Levels by RT-qPCR: Part I15. Analysis of gst::egfp mRNA
Levels by RT-qPCR: Part II 16. Analysis of gst::egfp mRNA Levels by
Semiquantitative RT-PCR: Part I 17. Analysis of gst::egfp mRNA Levels by
Semiquantitative RT-PCR: Part II
Part V: Modulation of Gene Expression18. Culturing Mammalian Cells19.
Transient Transfection of Mammalian Cells20. RNAi-Mediated Knockdown of
EGFP: Part I 21. RNAi-Mediated Knockdown of EGFP: Part II22. RNAi-Mediated
Knockdown of EGFP: Part III23. CRISPR-Mediated Knockout of EGFP: Part I24.
CRISPR-Mediated Knockout of EGFP: Part II 25. Advanced CRISPR: Part I 26.
Advanced CRISPR: Part II27. Interim Laboratory Session 28. Advanced CRISPR:
Part IV 29. Advanced CRISPR: Part V30. Advanced CRISPR: Part VI
AppendixA: Equipment B: Prep List C: Preparation of Competent Escherichia
coli Cells D: Pre-Lab Questions
Oriented; Practicing With Micropipettes 2. Purification and Digestion of
Plasmid (Vector) DNA3. Completion of Vector Preparation and Polymerase
Chain Reaction Amplification of egfp 4. Preparation of Insert DNA (egfp)
PCR Product5. DNA Ligation and Transformation of Escherichia coli
Part II: Screening TransformantsAdvanced Alternatives Within Part II6.
Screening of Transformants, Part I 7. Screening of Transformants, Part II8.
Analysis of DNA Sequence From a Positive Clone, Part II
Part III: Expression, Detection, and Purification of Recombinant Proteins
from BacteriaAdvanced Alternatives Within Part III 9. Expression of Fusion
Protein from Positive Clones, SDS_PAGE and Western Blot: Part I 10.
Expression of Fusion Protein from Positive Clones, SDS_PAGE, and Western
Blot: Part II 11. Extraction of Recombinant Protein From Escherichia coli
Using a Glutathione Affinity Column12. Analysis of Purification Fractions
Part IV: Analysis of mRNA Levels13. Total RNA Purification14. Analysis of
gst::egfp mRNA Levels by RT-qPCR: Part I15. Analysis of gst::egfp mRNA
Levels by RT-qPCR: Part II 16. Analysis of gst::egfp mRNA Levels by
Semiquantitative RT-PCR: Part I 17. Analysis of gst::egfp mRNA Levels by
Semiquantitative RT-PCR: Part II
Part V: Modulation of Gene Expression18. Culturing Mammalian Cells19.
Transient Transfection of Mammalian Cells20. RNAi-Mediated Knockdown of
EGFP: Part I 21. RNAi-Mediated Knockdown of EGFP: Part II22. RNAi-Mediated
Knockdown of EGFP: Part III23. CRISPR-Mediated Knockout of EGFP: Part I24.
CRISPR-Mediated Knockout of EGFP: Part II 25. Advanced CRISPR: Part I 26.
Advanced CRISPR: Part II27. Interim Laboratory Session 28. Advanced CRISPR:
Part IV 29. Advanced CRISPR: Part V30. Advanced CRISPR: Part VI
AppendixA: Equipment B: Prep List C: Preparation of Competent Escherichia
coli Cells D: Pre-Lab Questions
Part I: Manipulation of DNAAdvanced Alternatives Within Part I 1. Getting
Oriented; Practicing With Micropipettes 2. Purification and Digestion of
Plasmid (Vector) DNA3. Completion of Vector Preparation and Polymerase
Chain Reaction Amplification of egfp 4. Preparation of Insert DNA (egfp)
PCR Product5. DNA Ligation and Transformation of Escherichia coli
Part II: Screening TransformantsAdvanced Alternatives Within Part II6.
Screening of Transformants, Part I 7. Screening of Transformants, Part II8.
Analysis of DNA Sequence From a Positive Clone, Part II
Part III: Expression, Detection, and Purification of Recombinant Proteins
from BacteriaAdvanced Alternatives Within Part III 9. Expression of Fusion
Protein from Positive Clones, SDS_PAGE and Western Blot: Part I 10.
Expression of Fusion Protein from Positive Clones, SDS_PAGE, and Western
Blot: Part II 11. Extraction of Recombinant Protein From Escherichia coli
Using a Glutathione Affinity Column12. Analysis of Purification Fractions
Part IV: Analysis of mRNA Levels13. Total RNA Purification14. Analysis of
gst::egfp mRNA Levels by RT-qPCR: Part I15. Analysis of gst::egfp mRNA
Levels by RT-qPCR: Part II 16. Analysis of gst::egfp mRNA Levels by
Semiquantitative RT-PCR: Part I 17. Analysis of gst::egfp mRNA Levels by
Semiquantitative RT-PCR: Part II
Part V: Modulation of Gene Expression18. Culturing Mammalian Cells19.
Transient Transfection of Mammalian Cells20. RNAi-Mediated Knockdown of
EGFP: Part I 21. RNAi-Mediated Knockdown of EGFP: Part II22. RNAi-Mediated
Knockdown of EGFP: Part III23. CRISPR-Mediated Knockout of EGFP: Part I24.
CRISPR-Mediated Knockout of EGFP: Part II 25. Advanced CRISPR: Part I 26.
Advanced CRISPR: Part II27. Interim Laboratory Session 28. Advanced CRISPR:
Part IV 29. Advanced CRISPR: Part V30. Advanced CRISPR: Part VI
AppendixA: Equipment B: Prep List C: Preparation of Competent Escherichia
coli Cells D: Pre-Lab Questions
Oriented; Practicing With Micropipettes 2. Purification and Digestion of
Plasmid (Vector) DNA3. Completion of Vector Preparation and Polymerase
Chain Reaction Amplification of egfp 4. Preparation of Insert DNA (egfp)
PCR Product5. DNA Ligation and Transformation of Escherichia coli
Part II: Screening TransformantsAdvanced Alternatives Within Part II6.
Screening of Transformants, Part I 7. Screening of Transformants, Part II8.
Analysis of DNA Sequence From a Positive Clone, Part II
Part III: Expression, Detection, and Purification of Recombinant Proteins
from BacteriaAdvanced Alternatives Within Part III 9. Expression of Fusion
Protein from Positive Clones, SDS_PAGE and Western Blot: Part I 10.
Expression of Fusion Protein from Positive Clones, SDS_PAGE, and Western
Blot: Part II 11. Extraction of Recombinant Protein From Escherichia coli
Using a Glutathione Affinity Column12. Analysis of Purification Fractions
Part IV: Analysis of mRNA Levels13. Total RNA Purification14. Analysis of
gst::egfp mRNA Levels by RT-qPCR: Part I15. Analysis of gst::egfp mRNA
Levels by RT-qPCR: Part II 16. Analysis of gst::egfp mRNA Levels by
Semiquantitative RT-PCR: Part I 17. Analysis of gst::egfp mRNA Levels by
Semiquantitative RT-PCR: Part II
Part V: Modulation of Gene Expression18. Culturing Mammalian Cells19.
Transient Transfection of Mammalian Cells20. RNAi-Mediated Knockdown of
EGFP: Part I 21. RNAi-Mediated Knockdown of EGFP: Part II22. RNAi-Mediated
Knockdown of EGFP: Part III23. CRISPR-Mediated Knockout of EGFP: Part I24.
CRISPR-Mediated Knockout of EGFP: Part II 25. Advanced CRISPR: Part I 26.
Advanced CRISPR: Part II27. Interim Laboratory Session 28. Advanced CRISPR:
Part IV 29. Advanced CRISPR: Part V30. Advanced CRISPR: Part VI
AppendixA: Equipment B: Prep List C: Preparation of Competent Escherichia
coli Cells D: Pre-Lab Questions







