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Alcohol misuse is a ravaging public health and social problem. Alcohol-dependence (AD) is a phase of alcohol misuse in which the drinker consumes excessive amount of alcohol and have a continuous urge to consume alcohol. Metabolomics is a scientific field which may provide a novel method for the diagnosis of AD by using a sensitive and specific technique such as nuclear magnetic resonance (NMR). In this study, we have shown that the applied plasma and urine metabolomics technique using NMR was able to identify novel biomarkers in plasma and urine which can be useful to diagnose AD in the future.