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In enzymology, a pentalenene synthase (EC 4.2.3.7) is an enzyme that catalyzes the chemical reaction. Hence, this enzyme has one substrate, 2-trans,6-trans-farnesyl diphosphate, and two products, pentalenene and diphosphate. This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on phosphates. The systematic name of this enzyme class is 2-trans,6-trans-farnesyl-diphosphate diphosphate-lyase (cyclizing, pentalenene-forming). This enzyme is also called pentalenene synthetase.