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This book explores the fundamentals of plant molecular biology and the applications of biotechnology. It describes DNA, RNA and proteins, and their regulatory roles in plant development. The book introduces key techniques such as sequencing, genome editing (CRISPR-Cas9) and in vitro culture, which can be used to improve crops (disease/stress resistance, nutritional quality).Current issues (climate change, food safety, GMO ethics) are addressed. Biotechnological success stories, such as golden rice (vitamin A) and Bt corn (insect resistance), are illustrated by case studies. Practical workshops and discussions stimulate learning. The book concludes with an outlook for the future, highlighting the importance of an interdisciplinary approach to sustainable agricultural development and resource conservation in the face of global challenges.