This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Peptides, and Scaffolds
This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Peptides, and Scaffolds
Part III: Peptides 1. Stapled Peptides for Intracellular Drug Targets 2. Mapping of vascular zip codes by phage display 3. Engineering cyclic peptide toxins 4. Peptide Discovery using Bacterial Display and Flow Cytometry
Part V: Scaffolds 5. Designed Ankyrin Repeat Proteins (DARPins): From research to therapy 6. Target-binding proteins based on the tenth human fibronectin type III domain (10Fn3) 7. Anticalins: small engineered binding proteins based on the lipocalin scaffold 8. T Cell Receptor Engineering 9. Engineering Knottins as Novel Binding Agents
Part VI: Pharmacokinetics 10. Practical Theoretic Guidance for the Design of Tumor Targeting Agents 11. Re-Engineering Biopharmaceuticals for Targeted Delivery Across the Blood-Brain Barrier 12. Engineering and Identifying Supercharged Proteins for Macromolecule Delivery into Mammalian Cells
Part III: Peptides 1. Stapled Peptides for Intracellular Drug Targets 2. Mapping of vascular zip codes by phage display 3. Engineering cyclic peptide toxins 4. Peptide Discovery using Bacterial Display and Flow Cytometry
Part V: Scaffolds 5. Designed Ankyrin Repeat Proteins (DARPins): From research to therapy 6. Target-binding proteins based on the tenth human fibronectin type III domain (10Fn3) 7. Anticalins: small engineered binding proteins based on the lipocalin scaffold 8. T Cell Receptor Engineering 9. Engineering Knottins as Novel Binding Agents
Part VI: Pharmacokinetics 10. Practical Theoretic Guidance for the Design of Tumor Targeting Agents 11. Re-Engineering Biopharmaceuticals for Targeted Delivery Across the Blood-Brain Barrier 12. Engineering and Identifying Supercharged Proteins for Macromolecule Delivery into Mammalian Cells
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