Protein Post-Translational Modifications

Methods and Protocols
Herausgegeben:Zhang, Xu; Chen, Rui; Li, Jianjun

• Gebundenes Buch

Produktbeschreibung

This detailed volume explores robust analytical workflows for post-translational modification (PTM) analysis in both basic and translational contexts. After brief foundational reviews of the field, the book delves into detailed experimental and computational protocols for traditional bottom-up strategies and top-down proteoform analysis, as well as PTM analysis of biotherapeutic proteins, reflecting the growing emphasis on PTMs as critical quality attributes in biologic drug development and regulation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and practical, Protein Post-Translational Modifications: Methods and Protocols serves as a foundational and forward-looking resource to support and inspire continued exploration in this rapidly evolving field.

Produktdetails

• Methods in Molecular Biology 3018
• Verlag: Springer, Berlin; Humana
• Erscheinungstermin: 15. März 2026
• Englisch
• ISBN-13: 9781071651650
• ISBN-10: 107165165X
• Artikelnr.: 75917891

Inhaltsangabe

An Overview of Mass Spectrometry
Based Methods to Analyze Post
Translational Modifications.
Post
Translational Modifications in Protein Therapeutics: Importance and Analytical Tools.
Decoding the SUMO Proteome: A Mass Spectrometry Workflow for Site
Specific Identification.
Computational Pipelines for Protein Ubiquitylation Analysis and Prediction.
Extensive Characterization of N
Glycosylation from Major Histocompatibility Complex with Hydrophilic Interaction Chromatography and Mass Spectrometry.
Endogenous Phosphopeptidomics Analysis by Using Mesoporous IMAC Materials.
O
and N
Phosphoproteomic Analysis Using Online Alkaline
pH Reversed
Phase Nanoelectrospray
Tandem Mass Spectrometry.
Phosphoproteomics Protocol for the Identification of Novel Biomarkers from Extracellular Vesicles of Cancerous Cells.
Mapping Cellular Protein Lysine Methylation Using Targeted
Mass Spectrometry.
Enrichment and Metaproteomic Analysis of Lysine Acetylation in Fecal Microbiome Samples.
Generation of High
Quality Succinyl Spectral Libraries for Improved Proteome
Wide Succinylome Analysis Using Data
Independent Acquisition.
Combining SDS
PAGE with Capillary Zone Electrophoresis
Tandem Mass Spectrometry for Top
Down Proteomics Analysis of Intact Histone Proteoforms.
PEPPI
SP3: A Gel
Based High
Resolution Sample Preparation Workflow for In
Depth Top
Down Analysis of Intact Proteoforms by Mass Spectrometry.
Profiling O
Acetylation in Sialoglycans Using MALDI
TOF and LC
MS/MS with Methylamidation and Permethylation Derivatization.
Purification of Protein
Free and Protein
Linked Poly(ADP
Ribose) from Biochemical Reactions and from Human Cells.
An NMR
Based Approach for Global Arginine Methylation Analysis.
Nanopore
Based Detection of Protein Post
Translational Modifications.
Mass Spectrometry Analysis of Biotherapeutic Monoclonal Antibody Glycosylation.
Glycoengineering of Monoclonal Antibodies with Homogeneous Glycan Isomers with Asymmetric Antennae.
Deamidation Analysis of Biotherapeutic Proteins by Capillary Electrophoresis Mass Spectrometry.