Proteomic Profiling and Analytical Chemistry: The Crossroads, Second Edition helps scientists without a strong background in analytical chemistry to understand principles of the multistep proteomic experiment necessary for its successful completion. It also helps researchers who do have an analytical chemistry background to break into the proteomics field. Highlighting points of junction between proteomics and analytical chemistry, this resource links experimental design with analytical measurements, data analysis, and quality control. This targeted point of view will help both biologists and…mehr
Proteomic Profiling and Analytical Chemistry: The Crossroads, Second Edition helps scientists without a strong background in analytical chemistry to understand principles of the multistep proteomic experiment necessary for its successful completion. It also helps researchers who do have an analytical chemistry background to break into the proteomics field. Highlighting points of junction between proteomics and analytical chemistry, this resource links experimental design with analytical measurements, data analysis, and quality control. This targeted point of view will help both biologists and chemists to better understand all components of a complex proteomic study.
The book provides detailed coverage of experimental aspects such as sample preparation, protein extraction and precipitation, gel electrophoresis, microarrays, dynamics of fluorescent dyes, and more. The key feature of this book is a direct link between multistep proteomic strategy and quality control routinely applied in analytical chemistry. This second edition features a new chapter on SWATH-MS, substantial updates to all chapters, including proteomic database search and analytical quantification, expanded discussion of post-hoc statistical tests, and additional content on validation in proteomics.
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Autorenporträt
Dr. Jerzy Silberring obtained a PhD in Chemistry in 1978 at Jagiellonian University, Kraków, Poland. He received a Docent of Biochemistry title at the University of Uppsala in 1991, and became a full professor of biochemistry at the Jagiellonian University in 2001. He spent 10 years at the Uppsala University and Karolinska Institute in Stockholm. He is the author of over 200 papers and reviews mainly on proteolytic enzymes, neuropeptides, neurobiology of pain and drug dependence, proteomics, and mass spectrometry. His investigations have contributed to our understanding of the regulation of neuropeptides and their fragments, discovery of novel peptides, and applications of mass spectrometry. He teaches biochemistry, neurobiology of behavior, and drug dependence at the AGH University of Science and Technology, Jagiellonian University, and the Centre of Polymer and Carbon Materials, Polish Academy of Sciences.
Inhaltsangabe
Preface 1. Introduction Jerzy Silberring and Pawel Ciborowski 2. Biomolecules Ariel Burns, Pawel Olszowy and Pawel Ciborowski 3. General Strategies for Proteomic Sample Preparation Piotr Suder, Peter Novak, Vladimir Havlicek and Anna Bodzon-Kulakowska 4. Protein Extraction and Precipitation Petr Novak and Vladimir Havlicek 5. Online and Offline Sample Fractionation Marek Smoluch, Przemyslaw Mielczarek, Anna Drabik and Jerzy Silberring 6. Immunoaffinity Depletion of High-Abundant Proteins for Proteomic Sample Preparation Jayme Wiederin and Pawel Ciborowski 7. Gel Electrophoresis Anna Drabik, Anna Bodzon-Kulakowska and Jerzy Silberring 8. Quantitative Measurements in Proteomics: Mass Spectrometry Anna Drabik and Jerzy Silberring 9. SWATH-MS: Data Acquisition and Analysis Kristin Frederick and Pawel Ciborowski 10. Top-Down Proteomics Cory Boone and Jiri Adamec 11. Proteomic Database Search and Analytical Quantification for Mass Spectrometry Melinda Wojtkiewicz, Jayme Wiederin and Pawel Ciborowski 12. Design and Statistical Analysis of Mass-Spectrometry-Based Quantitative Proteomics Data Fang Yu, Fang Qiu and Jane Meza 13: Principles of Analytical Validation Jo-Ellyn McMillan 14.Validation in Proteomics and Regulatory Affairs Jerzy Silberring, Melinda Wojtkiewicz and Pawel Ciborowski
Preface 1. Introduction Jerzy Silberring and Pawel Ciborowski 2. Biomolecules Ariel Burns, Pawel Olszowy and Pawel Ciborowski 3. General Strategies for Proteomic Sample Preparation Piotr Suder, Peter Novak, Vladimir Havlicek and Anna Bodzon-Kulakowska 4. Protein Extraction and Precipitation Petr Novak and Vladimir Havlicek 5. Online and Offline Sample Fractionation Marek Smoluch, Przemyslaw Mielczarek, Anna Drabik and Jerzy Silberring 6. Immunoaffinity Depletion of High-Abundant Proteins for Proteomic Sample Preparation Jayme Wiederin and Pawel Ciborowski 7. Gel Electrophoresis Anna Drabik, Anna Bodzon-Kulakowska and Jerzy Silberring 8. Quantitative Measurements in Proteomics: Mass Spectrometry Anna Drabik and Jerzy Silberring 9. SWATH-MS: Data Acquisition and Analysis Kristin Frederick and Pawel Ciborowski 10. Top-Down Proteomics Cory Boone and Jiri Adamec 11. Proteomic Database Search and Analytical Quantification for Mass Spectrometry Melinda Wojtkiewicz, Jayme Wiederin and Pawel Ciborowski 12. Design and Statistical Analysis of Mass-Spectrometry-Based Quantitative Proteomics Data Fang Yu, Fang Qiu and Jane Meza 13: Principles of Analytical Validation Jo-Ellyn McMillan 14.Validation in Proteomics and Regulatory Affairs Jerzy Silberring, Melinda Wojtkiewicz and Pawel Ciborowski
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