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E. coli O157:H7 is considered as an important pathogen of diarrhea in adult dogs and puppies. Dogs and puppies can be regarded as important reservoirs for E. coli O157:H7 which is one of the main causes of diarrhea and other diseases in humans. The large part of this book is about the description of pathogenesis of E. coli O157:H7 in puppies and their effect on different tissues. Also, this book gives an idea about diagnosis of the most virulence factors of this pathogen by Conventional and Real time PCR.