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Malaria is an infectious disease caused by by species of parasites of genus plasmodium that affect s human infecting erythrocytes. Malaria caused by p.falciparum is the most deadly manner. About 80-90 percent of deaths are estimated to occur in African region with children less then five years of age and pregnant women (WHO-2012). As the second most popular country in the world with a population exceeding one billion people, India's public health faces many challenges including implementation of surveillance program to accurately estimate and control the national malaria burden. In this book, we try to study the effectiveness of SP as first line chemotherapy for uncomplicated p.falciparum. And identify the "recrudescence" or "re-infection" of p.falciparum malaria using molecular markers. Screening of mutations profile of DHFR and DHPS of p.falciparum identified in different location of West Bengal. Finally study the association of gene mutations with clinical response to SP.