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This study focuses on the synthesis of poly methyl methacrylate (MMA) and poly methyl methacrylate-co-2-hydroxyethyl methacrylate (HEMA) with the aim of producing biocompatible copolymeric nanoparticles within the nanoscale range (under 50 nm) through the differential microemulsion polymerization method. Microemulsion polymerization is a process that facilitates the solubilization of bioactive compounds and is noted for its spontaneous formation, simplicity in production, compatibility with various additives, stability across a broad temperature spectrum, and low viscosity. Furthermore, the differential approach allows for the utilization of minimal emulsifier quantities while enhancing the solid content. A range of emulsifiers, such as sodium dodecyl sulfate (SDS) and biocompatible options like sodium alginate (SA) and polyvinyl pyrrolidone (PVP), were employed to investigate the microemulsion polymerization process.