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To prepare such a Major Practical Manual for students and teachers as well as for those people who have scientific attitude and interest for the subject. The current Major practical syllabus of zoology has been framed from Periyar University as well as State level. As there are no such Manual which cover diagrams only but we have insert the original specimen picture were included. We thought to prepare such a Major Practical Manual for students and Professor as well as for those people who have scientific aptitude and interest for the subject and support to prepare semester examination. The Manual is richly illustrated with figures and original photographs. First aid, precautionary methods and safely rules and regulations and also included specific guidelines, how to handling the equipment in the laboratory.