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The present book, 'The Wonder Climbers,' explores the isolation of medicinally active ingredients from two plant species, Glycyrrhiza glabra and Tinospora cordifolia, aiming to study their useful phytochemical constituents. The research involves in vitro culture, isolation, identification, and quantification of primary metabolites such as carbohydrates, starch, proteins, lipids, phenols, chlorophyll, and carotenoids, along with secondary metabolites such as steroidal sapogenin and rotenoids. Various chromatographic and spectroscopic techniques are employed for this purpose. Furthermore, the book discusses the biological activity of the isolated compounds, including antibacterial, antifungal, and antioxidant properties. The antimicrobial activity is validated through established protocols for antibacterial and antifungal bioassays, while antioxidant activity is assessed using recognized methods. This comprehensive study sheds light on the pharmacologically valuable constituents of the selected plant species.