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Enzymes are the bioactive compounds that have focused attention. The demand for microbial enzymes in industrial fields is increasing day by day due to their applications in clean and cost effective biotechnological processes. A halo-tolerant and alkaliphilic bacterium, Kh-30-2, was isolated from saline soil of the desert of Kutch, Gujarat. The organism secreted a range of extracellular enzymes including protease, cellulase and lipase with 10% NaCl and pH 9. It secreted alkaline protease optimally with 0.5 % w/v, peptone and yeast extract. The enzyme was partially purified by ammonium sulphate…mehr

Produktbeschreibung
Enzymes are the bioactive compounds that have focused attention. The demand for microbial enzymes in industrial fields is increasing day by day due to their applications in clean and cost effective biotechnological processes. A halo-tolerant and alkaliphilic bacterium, Kh-30-2, was isolated from saline soil of the desert of Kutch, Gujarat. The organism secreted a range of extracellular enzymes including protease, cellulase and lipase with 10% NaCl and pH 9. It secreted alkaline protease optimally with 0.5 % w/v, peptone and yeast extract. The enzyme was partially purified by ammonium sulphate precipitation with 4.84 fold purification and 7.42% yield. The enzyme displayed optimum activity at 60°C temperature and pH 10. Significant stability of the enzyme with detergent and cations reflected its potential as a promising detergent additive.
Autorenporträt
Dr Jignasha Thumar pracuje jako adiunkt (urz¿dnik II klasy w s¿übie edukacyjnej stanu Gud¿arat) w dziedzinie mikrobiologii w Government Science College w Gandhinagar w stanie Gud¿arat w Indiach. Jest laureatk¿ nagrody Bio-CARe dla kobiet naukowców przyznawanej przez rz¿d Indii. Jej zainteresowania badawcze koncentruj¿ si¿ na ekstremofilnych promieniowcach i ich bioaktywnych metabolitach.