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3D printing, also known as additive manufacturing, has gained widespread recognition for its applications in tissue engineering, art, and architectural modeling. The focus on 3D printing in tissue engineering is growing among researchers due to its ability to manufacture highly accurate and personalized scaffolds rapidly. Scaffolds, crucial for tissue regeneration and repair in regenerative medicine, are crafted from various materials such as natural, synthetic, and copolymer polymers. These scaffolds provide a supportive structure for cell nurturing and differentiation, facilitating the regeneration of damaged tissues or organs. This paper comprehensively explores scaffold materials, fabrication methods, and the role of 3D printing in tissue engineering applications, emphasizing the crucial interplay between material properties, scaffold architecture, and the specific requirements of the target tissue for successful regeneration and integration of functional tissue constructs.