Tissue Fixation and Processing - Uppala, Divya;Balla, Hyandavi;Pamidimukkala, Sahithi
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This comprehensive manual provides an in-depth exploration of tissue fixation, processing, and embedding techniques essential in histopathology and biomedical research. Beginning with the fundamental concepts of autolysis and fixation, the book delves into the chemistry and classification of various fixatives including aldehydes, oxidizing agents, protein denaturants, and cross-linking compounds highlighting their mechanisms, applications, and impact on tissue morphology and staining properties. It further addresses the physical and chemical factors affecting fixation, such as pH, temperature,…mehr

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Produktbeschreibung
This comprehensive manual provides an in-depth exploration of tissue fixation, processing, and embedding techniques essential in histopathology and biomedical research. Beginning with the fundamental concepts of autolysis and fixation, the book delves into the chemistry and classification of various fixatives including aldehydes, oxidizing agents, protein denaturants, and cross-linking compounds highlighting their mechanisms, applications, and impact on tissue morphology and staining properties. It further addresses the physical and chemical factors affecting fixation, such as pH, temperature, penetration rate, and osmolality. Detailed discussions cover both conventional and advanced methods like microwave fixation and the use of secondary fixatives for enhanced preservation. The book also provides extensive guidance on tissue processing stages, including dehydration, clearing, impregnation, and embedding using paraffin, celloidin, resins, and ester wax. Special sections include techniques for photomicrography, image analysis, and tumor marker identification, offering valuable insights for diagnostic and research applications.
Autorenporträt
Dr. Divya Uppala is working as Professor and Head of the Department in Oral Pathology and Microbiology in GITAM Dental College and Hospital. Dr. Hyandavi Balla and Dr. Sahithi Pamidimukkala are working as Assistant Professors in GITAM Dental College, Hospital in Oral Pathology and Microbiology, Conservative Dentistry and Endodontics respectively.