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Abiotic stress severely affects the growth, quality, and ornamental value of ornamental plants, which are key to horticultural ecosystems and urban greening. This Special Issue of Horticulturae focuses on "Tolerance and Response of Ornamental Plants to Abiotic Stress", showcasing the latest research on how ornamental plants adapt to stressors like heavy metal pollution, saline-alkali environments, extreme temperatures, and abnormal light, as well as the underlying regulatory mechanisms. All papers in this Reprint have passed strict peer review by experts and Guest Editors, ensuring high academic quality, and were published in Horticulturae in full open access format for knowledge sharing. It contains eleven publications, including one editorial, nine research articles, and one review article, which provide a representative and valuable collection of studies on the tolerance mechanisms and response patterns of ornamental plants under various abiotic stresses. This Reprint summarizes current progress in ornamental plant abiotic stress tolerance, provides references for cultivating stress-resistant varieties and improving adaptability in harsh environments, serves as a valuable resource for students, researchers, and horticultural technicians in related fields, and highlights directions for future research.