Michael W. Quick

Transmembrane Transporters

Ed. by Michael W. Quick

• Gebundenes Buch

Produktbeschreibung

This comprehensive text provides readers with a state-of-the-art molecule update on transmembrane transporters, focusing on the methodological approaches currently employed to better understand how transporters work and how they can be applied in cutting-edge therapies. This book is organized by experimental approaches, including chapters devoted to: informatics, expression strategies, high-throughput screening, molecular genetics, structural analyses (NMR, crystallization, freeze fracture, and chimeras), and functional analyses (imaging, electrophysiology, and microdialysis).

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A must-have far-reaching text that provides readers with a state-of-the-art molecule update on transmembrane transporters, focusing on the methodological approaches currently employed to better understand how transporters work and how they can be used in cutting edge therapies. Each chapter begins with an overview of the importnat biological questions presently being considered in their field, then presents scientific approaches to address these questions. In explaining approaches, the authors cover bench-top protocols, conceptual frameworks, data obtained, and pitfalls common to the techniques.

Produktdetails

• Receptor Biochemistry and Methodology
• Verlag: Wiley & Sons; Wiley-Liss
• Seitenzahl: 316
• Erscheinungstermin: 9. September 2002
• Englisch
• Abmessung: 262mm x 181mm x 20mm
• Gewicht: 772g
• ISBN-13: 9780471065135
• ISBN-10: 0471065137
• Artikelnr.: 11081356

Autorenporträt

Michael W. Quick received his Ph.D. from Emory University in 1992. Following postdoctoral training at the California Institute of Technology, he joined the faculty at the University of Alabama at Birmingham. His work focuses on the mechanisms by which neurotransmitter transporter function and expression are regulated.

Inhaltsangabe

Series Preface.
Preface.
Contributors.
Families of Transporters and Their Classification (M. Saier).
Yeast as a Model System for Studying Glucose Transport (E. Boles).
Neurotransmitter Transporters of Drosophila (M. Burg & W. Pak).
Transgenic Mice in Monoamine Transporter Research (S. Jones).
Searching for Novel Genetic Variation in Neurotransmitter Transporters (R.
Blakely & A. Belous).
Nonviral Gene Transfer Allows Up- and Down-Expression of the Brain
Serotonin Transporter with Functional Consequences (M. Martres, et al.).
Methods in Studying the Regulation and Trafficking of Transmembrane
Transporters (S. Deken, et al.).
Chemical Modification Strategies for Structure-Function Studies (G.
Rudnick).
Methanethiosulfonate Reagent Accessibility Studies, Cysteine-Scanning
Mutagenesis, Protein Overexpression, and Functional Reconstitution: A
Strategy for Studying the Structure/Function Relationships within the
Mitochondrial Citrate Transport Protein (R. Kaplan).
Peptide Mapping of Dopamine Transporter Ligand and Substrate Interaction
Sites (M. Lowe, et al.).
Mass Spectrometry of Membrane Transport Proteins (J. Whitelegge, et al.).
Amperometric Recording of Amphetamine-Induced Dopamine Efflux (D. Sulzer,
et al.).
Voltage Clamp and Fluorometric Techniques for Studying Glutamate
Transporter Function (A. Tzingounis, et al.).
Studies of Glial Glutamate Transporters in Hippocampal Microcultures (S.
Mennerick, et al.).
Imaging Monoamine Transporters in the Brain (H. Kung & M. Kung).
Measuring and Modeling the Spatiotemporal Profile of GABA at the Synapse
(L. Overstreet, et al.).
Index.

Rezensionen

"...superb, wide-ranging, and up-to-date volume is highly recommended for researchers, college instructors, and graduate and advanced undergraduate students..." ( The Quarterly Review of Biology , Vol. 7, March 2004)
"...introduces many technologies to be used in future studies....people, who are interested in transporter research, can get new ideas for next research from this book." ( Pharmaceutical Research , Vol. 20, No. 12, December 2003)