Bringing this best-selling textbook right up to date, the new edition uniquely integrates the theories and methods that drive the fields of biology, biotechnology and medicine, comprehensively covering both the techniques students will encounter in lab classes and those that underpin current key advances and discoveries. The contents have been updated to include both traditional and cutting-edge techniques most commonly used in current life science research. Emphasis is placed on understanding the theory behind the techniques, as well as analysis of the resulting data. New chapters cover…mehr
Bringing this best-selling textbook right up to date, the new edition uniquely integrates the theories and methods that drive the fields of biology, biotechnology and medicine, comprehensively covering both the techniques students will encounter in lab classes and those that underpin current key advances and discoveries. The contents have been updated to include both traditional and cutting-edge techniques most commonly used in current life science research. Emphasis is placed on understanding the theory behind the techniques, as well as analysis of the resulting data. New chapters cover proteomics, genomics, metabolomics, and bioinformatics, as well as data analysis and visualisation. Using accessible language to describe concepts and methods, and with a wealth of new in-text worked examples to challenge students' understanding, this textbook provides an essential guide to the key techniques used in current bioscience research.
Foreword Keith Wilson and John Walker Preface Samuel Clokie and Andreas Hofmann Tables and resources Andreas Hofmann 1. Life science studies Samuel Clokie and Andreas Hofmann 2. Basic principles Parisa Amani and Andreas Hofmann 3. Cell culture techniques Anwar Baydoun 4. Recombinant DNA techniques and molecular cloning Ralph Rapley 5. Preparative biochemistry Samuel Clokie 6. Electrophoresis Ralph Rapley 7. Immunochemical techniques Katja Fischer 8. Flow cytometry John Grainger and Joanne Konkel 9. Radioisotope techniques Robert Slater 10. Clinical biochemistry Gillian Rumsby 11. Microscopy Stephen Paddock 12. Centrifugation Kay Ohlendieck and Stephen Harding 13. Spectroscopic techniques Anne Simon and Andreas Hofmann 14. Basic techniques probing molecular structure and interactions Anne Simon and Joanne Macdonald 15. Mass spectrometric techniques Sonja Hess and James MacRae 16. Fundamentals of bioinformatics Cinzia Cantacessi and Anna Protasio 17. Fundamentals of cheminformatics Paul Taylor 18. The Python programming language Timothy Stevens 19. Processing and visualisation of experimental data Jean-Baptiste Cazier 20. Fundamental genome sequencing and annotation Pasi Korhonen and Robin Gasser 21. Fundamentals of proteomics Sonja Hess and Michael Weiss 22. Fundamentals of metabolomics James MacRae 23. Enzymes and receptors Megan Cross and Andreas Hofmann 24. Drug discovery and development David Camp.
Foreword Keith Wilson and John Walker Preface Samuel Clokie and Andreas Hofmann Tables and resources Andreas Hofmann 1. Life science studies Samuel Clokie and Andreas Hofmann 2. Basic principles Parisa Amani and Andreas Hofmann 3. Cell culture techniques Anwar Baydoun 4. Recombinant DNA techniques and molecular cloning Ralph Rapley 5. Preparative biochemistry Samuel Clokie 6. Electrophoresis Ralph Rapley 7. Immunochemical techniques Katja Fischer 8. Flow cytometry John Grainger and Joanne Konkel 9. Radioisotope techniques Robert Slater 10. Clinical biochemistry Gillian Rumsby 11. Microscopy Stephen Paddock 12. Centrifugation Kay Ohlendieck and Stephen Harding 13. Spectroscopic techniques Anne Simon and Andreas Hofmann 14. Basic techniques probing molecular structure and interactions Anne Simon and Joanne Macdonald 15. Mass spectrometric techniques Sonja Hess and James MacRae 16. Fundamentals of bioinformatics Cinzia Cantacessi and Anna Protasio 17. Fundamentals of cheminformatics Paul Taylor 18. The Python programming language Timothy Stevens 19. Processing and visualisation of experimental data Jean-Baptiste Cazier 20. Fundamental genome sequencing and annotation Pasi Korhonen and Robin Gasser 21. Fundamentals of proteomics Sonja Hess and Michael Weiss 22. Fundamentals of metabolomics James MacRae 23. Enzymes and receptors Megan Cross and Andreas Hofmann 24. Drug discovery and development David Camp.
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