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This book reports on cutting-edge research and best practices in the broad field of biomedical engineering. Based on the XLVIII Mexican Conference on Biomedical Engineering, CNIB 2025, held on September 11-13, 2025 in Nuevo León, Monterrey, Mexico, this second volume of the proceedings covers research topics in biomechanics, materials and engineering design and manufacturing, with applications in prostheses design and development, tissue engineering, medical device assessment and healthcare management. All in all, this book provides a timely snapshot on state-of-the-art achievements in biomedical engineering and current challenges in the field. It addresses both researchers and professionals, and it is expected to foster future collaborations between the two groups, as well as international collaborations.